The acute effects of triacylglycerol structure of palmitic acid rich fats on chylomicron composition in lean subjects and those with the Metabolic Syndrome.

Not Applicable

Completed

• Conditions: Metabolic Syndrome; Metabolic and Endocrine - Metabolic disorders

• Registration Number: ACTRN12615000147583
• Lead Sponsor: Auckland UniServices

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2015-02-17
• Last Update Posted: 13 January 2020

Recruitment & Eligibility

• Status: Completed
• Sex: Male
• Target Recruitment: 40

Inclusion Criteria

Group 1:
45-60 years old
BMI of 18-25 kg/m2
Healthy (no current or past history of the Metabolic Syndrome as defined by the International Diabetes Federation)

Group 2:
45-60 years old
BMI greater than 25 kg/m2
Metabolic Syndrome as defined by the International Diabetes Federation: central obesity (waist circumference greater than/equal to 94cm PLUS any two of:
* raised TG concentrations (greater than/equal to 1.7 mmol/L or undergoing treatment)
* reduced HDL-C (<1.03 mmol/L or undergoing treatment)
* raised blood pressure (greater than/equal to 130/85 mmHg or undergoing treatment)
* raised fasting plasma glucose (greater than/equal to 5.6 mmol/L or previously diagnoses T2DM)

Exclusion Criteria

History of CVD disease/complications (myocardial infarction, angina, stroke)
History of cancer
Pre-existing diabetes
Self reported alcohol intake exceeding a moderate intake (>28 units/week)
Abnormal liver function or haematology

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Difference in chylomicron TAG concentration measured by enzymatic immunoassay after test fat ingestion relative to control fat[Baseline and hourly for 5 hours post-meal at each visit];Differences in chylomicron TAG composition and regio-isomerisation measured by lipidomic analysis after test fat ingestion relative to control fat[Baseline and hourly for 5 hours post-meal at each visit];Differences in chylomicron particle size measured by dynamic light scattering after test fat ingestion relative to control subjects[Baseline and hourly for 5 hours post-meal at each visit]

Secondary Outcome Measures

Name	Time	Method
Differences in immune cell activation by peripheral blood mononuclear cell gene expression of inflammatory cytokines measured by quantitative polymerase chain reaction after test fat ingestion relative to control subjects[Baseline, 2 hours and 4 hours post-meal at each visit];Differences in plasma metabolites (including acyl-carnitines to quantify lipid peroxidation) measured by GC-MS after test fat ingestion relative to control fat[Baseline and hourly for 5 hours post-meal at each visit]