Clinical and Molecular Biological Data Collection and Analysis in Patients With Traumatic Brain Injury (TBI)
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 200
- 试验地点
- 1
- 主要终点
- Accurate assessment of patients' neurological condition
研究概览
简要总结
Clinical evaluation is crucial in the clinical diagnosis, treatment, and prognosis prediction in patients with traumatic brain injury (TBI). However, the existing evaluation systems are not perfect, because many factors are not taken into account, for example, there is a lack of molecular diagnostic criteria for evaluating patients with TBI. We attempt to collect the patient's clinical data and combine it with neuroimaging, as well as molecular biomarkers generated by single-cell sequencing to assess their neurological status and outcome. The clinical and molecular data collection and analysis will be helpful to evaluate the patient's neurological condition and predict the patient's outcome more accurately.
详细描述
Specifically, eligible patients will be treated surgically. In one group, injured brain tissue removed during decompression and resection will be preserved in a tissue preservation solution. In another group, brain tissue in the surgical trajectory during surgical resection of the brain lesion (for example, gliomas, meningiomas, schwannomas) will be also preserved in the solution. Then these tissues will be analyzed through high-throughput single-cell sequencing and bioinformatics analysis. In order to obtain the most changed biomarkers, we will pick up the top 10 cell subgroups with 10 expression biomarkers in each subgroup to compare them with the results analyzed in the mice brain tissue. The target biomarkers will be validated in the cerebrospinal fluid (CSF) and blood in patients with TBI for the patient's neurological status evaluation and outcome prediction. At the same time, the patient's pre- and postoperative clinical data (including, gender, age, GCS score, pupil light reflection, CT results, etc) will also be collected. A regression model will be generated to estimate the relationship between the patient's neurological status and these independent variables. In addition, the association between a patient's prognosis and these factors will also be analyzed. ROC curve will be used to compare our regression model to the established GCS score scale and Marshall CT grading.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •CT/MRI confirmed TBI;
- •The time from onset to the emergency room (ER ) within 12h;
- •The systolic pressure (SBP) ≥ 90 mmHg;
- •Patients willing to participate in this study with signed informed consent.
排除标准
- •Patients who are not suitable for surgery (GCS score 3 points, dilated and fixed bilateral pupils, pregnant women, etc.);
- •Pre-injury life expectancy ≤ 1 year (malignant tumor);
- •Previous history of acute myocardial infarction, abnormal immune function, and blood-related diseases;
- •without informed consent.
结局指标
主要结局
Accurate assessment of patients' neurological condition
时间窗: 2022.09-2023.12
Using a regression model to explore the relationship between a patient's neurological condition and clinical and molecular biological data
次要结局
- Predicting patients' outcome accurately(2022.09-2023.12)
研究者
Hao Chen, MD, PhD
Associate Clinical Professor, Supervisor
Shanghai 6th People's Hospital