Evaluation the effect of microfluidic and density gradient centrifugation methods on sperm parameters and sperm DNA, fertilization rate, embryo quality and expression of genes affecting fertility in patients with fertilization failure compared with control in ICSI cycles.
Not Applicable
Recruiting
- Conditions
- Fertilization failure.
- Registration Number
- IRCT20190523043676N1
- Lead Sponsor
- Iran University of Medical Sciences
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Male
- Target Recruitment
- 20
Inclusion Criteria
A woman aged between 18 and 35 years old
Fertilization failure after at least one ICSI cycle
28> Female BMI> 20
Normal sperm parameters
No smoking in couples
Agonist ovulation stimulation protocol
Eggs in M2 stage
Exclusion Criteria
Severe endometriosis
Varicocele in men
Severe PCO
Study & Design
- Study Type
- interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Fertilization failure, sperm preparation methods. Timepoint: Fertilization failure: Diagnosis of file patients before entering the study. Methods of sperm preparation: After sampling, semen is divided into two groups and washed with two methods of preparation.?. Method of measurement: Fertilization failure: Based on patient records, sperm preparation methods: DGC and microfluidics.
- Secondary Outcome Measures
Name Time Method Gene expression changes in studied groups. Timepoint: At the end of the study, after freeze-thawed semen. Method of measurement: Real-time PCR.;Sperm parameters. Timepoint: after semen sampling (before preparation) and after both microfluidic and DGC preparation methods. Method of measurement: Semen Analysis( Using Meckler Lam and Light Microscope).;DFI sperm. Timepoint: semen sampling (before preparation) and after both microfluidic and DGC preparation methods. Method of measurement: Halo Method.;Fertilization rate (number of embryos obtained). Timepoint: 18 to 20 hours after ICSI. Method of measurement: Light Microscope.;Quality of obtained embryos. Timepoint: after 48 and 72 hours of ICSI. Method of measurement: Light microscope.