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Serum Interleukin -21 Level in Patients With Severe Adverse Cutaneous Drug Reaction.

Not Applicable
Conditions
Adverse Cutaneous Reaction to Alternative Medical Therapy
Interventions
Diagnostic Test: serum interleukin 21
Diagnostic Test: Complete Blood Picture
Diagnostic Test: Liver function test
Diagnostic Test: Random Blood Sugar
Diagnostic Test: Erythrocyte Sedimentation Rate
Diagnostic Test: Kidney function tests
Registration Number
NCT03166241
Lead Sponsor
Assiut University
Brief Summary

Adverse cutaneous drug reactions are undesirable and typically unanticipated reactions independent of the intended therapeutic purpose of a medication. It may be either immunologic (eg, drug allergy) or non-immunologic. Adverse cutaneous drug reaction produce a wide range of clinical manifestations such as pruritus, maculopapular eruptions, urticaria, angioedema, phototoxic and photo allergic reactions, fixed drug reactions, erythema multiforme,vesiculobullous reactions (eg, Stevens-Johnson syndrome and toxic epidermal necrolysis) and serum sickness .They must be considered in the differential diagnosis of sudden symmetric eruption

Detailed Description

Erythema multiforme is an acute immune mediated disorder It is a type IV hypersensitivity reaction leading to dermal vasculitis. Erythema multiforme major usually as a result of medications such as sulphonamides, non-steroidal anti-inflammatories and penicillin and there is skin and mucosal involvement.

Steven-Johnson syndrome (SJS) and Toxic Epidermal Necrolysis (TEN) are considered a spectrum of acute life-threatening mucocutaneous reactions that differ only in severity. Both diseases are characterized by mucous membrane and skin involvement, are often caused by medications and are collectively known as epidermal necrolysis or scalded skin syndrome.Stevens-Johnson syndrome (SJS) is classified as an epidermal loss \<10% of the body surface area.Toxic Epidermal Necrolysis (TEN) is indicated by \>30% body surface area erosion. The range of epidermal loss between 10% and 30% is called Stevens-Johnson syndrome-Toxic Epidermal Necrolysis (SJS-TEN) overlap. Severity of illness score \[Score of Toxic Epidermal Necrolysis(TEN) \] has been devised to predict prognosis in patients with Epidermal Necrolysis.This scoring system addresses 7 prognostic factors: age, malignancy, heart rate,Body Surface Area involved, serum urea, serum glucose and serum bicarbonate levels.

Interleukin-21 regulates both innate and adaptive immune responses and it is not only has key roles in antitumour and antiviral responses that promote the development of autoimmune diseases and inflammatory disorders. It is recently discovered member of the type 1 cytokine family which is produced by activated clusters of differentiation 4+ T cells ,Natural killer cells and follicular helper T cells.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
30
Inclusion Criteria
  • 20 patients presenting with adverse cutaneous drug reaction (Erythema multiforme, SJS and TEN )
  • Patients with definite drug history.
  • Both sex will be included.
Exclusion Criteria
  • Patients with a history of topical or systemic treatment (corticosteroids, intralesional steroid injection, immunosuppressive therapy).
  • Patients within 4 weeks of the study.
  • Patients receiving phototherapy within 6 months of the study.
  • Diabetic patients
  • Anaemic patients
  • Thyroid disorders,
  • Chronic liver or Renal diseases
  • Atopy and Parathyroid disorders.
  • Patients with known autoimmune diseases or cancer.
  • Pregnant or lactating womens.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
study groupLiver function testmeasure serum interleukin 21 level in patients with severe adverse drug reaction who show change in serum interleukin 21 before and after therapy the following investigation will be done at the begning of the study to patients: Complete Blood Picture Erythrocyte Sedimentation Rate Random blood sugar Liver function tests Kidney function tests
study groupRandom Blood Sugarmeasure serum interleukin 21 level in patients with severe adverse drug reaction who show change in serum interleukin 21 before and after therapy the following investigation will be done at the begning of the study to patients: Complete Blood Picture Erythrocyte Sedimentation Rate Random blood sugar Liver function tests Kidney function tests
study groupErythrocyte Sedimentation Ratemeasure serum interleukin 21 level in patients with severe adverse drug reaction who show change in serum interleukin 21 before and after therapy the following investigation will be done at the begning of the study to patients: Complete Blood Picture Erythrocyte Sedimentation Rate Random blood sugar Liver function tests Kidney function tests
control groupserum interleukin 21compare serum interleukin 21 level in patients with severe adverse drug reaction and healthy control subjects
study groupserum interleukin 21measure serum interleukin 21 level in patients with severe adverse drug reaction who show change in serum interleukin 21 before and after therapy the following investigation will be done at the begning of the study to patients: Complete Blood Picture Erythrocyte Sedimentation Rate Random blood sugar Liver function tests Kidney function tests
study groupComplete Blood Picturemeasure serum interleukin 21 level in patients with severe adverse drug reaction who show change in serum interleukin 21 before and after therapy the following investigation will be done at the begning of the study to patients: Complete Blood Picture Erythrocyte Sedimentation Rate Random blood sugar Liver function tests Kidney function tests
study groupKidney function testsmeasure serum interleukin 21 level in patients with severe adverse drug reaction who show change in serum interleukin 21 before and after therapy the following investigation will be done at the begning of the study to patients: Complete Blood Picture Erythrocyte Sedimentation Rate Random blood sugar Liver function tests Kidney function tests
Primary Outcome Measures
NameTimeMethod
the patients with severe adverse cutaneous drug reaction who show change in serum interleukin 21 before and after therapyone month

blood sample will be taken from patients

Secondary Outcome Measures
NameTimeMethod
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