Dairy and Plant amino acid uptake - An explorative study

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 12

Inclusion Criteria

• Apparently healthy men and women;
• Age >=18 and <=40 years;
• Body mass index (BMI) >=18.5 and <=30 kg/m2 ;
• Having veins suitable for blood sampling via a catheter (judged by study
nurse/ medical doctor).

Exclusion Criteria

• Any self-reported metabolic, gastrointestinal, inflammatory or chronic
disease (such as anemia, diabetes, hepatitis, cardiovascular disease);
• Having a history of medical or surgical events that may significantly affect
the study outcome, including: Inflammatory bowel disease, hepatitis,
pancreatitis, ulcers, gastrointestinal or rectal bleeding; major
gastrointestinal tract surgery such as gastrectomy, gastroenterostomy, or bowel
resection; known or suspected gastrointestinal disorders, colon or GI tract
cancer;
• Anaemia (Haemoglobin (Hb) values <7.5 mmol/L for women and <8.5 mmol/L for
men), as assessed by finger prick blood during screening visit;
• Having a food allergy, cow*s milk protein, soy or bean protein allergy,
favism (G6PD-deficiency), and/or lactose intolerance (self-reported);
• Use of glucose lowering drugs, insulin;
• Use of medication that may impact gastric emptying (e.g. gastric acid
inhibitors or laxatives);
• Use of antibiotic treatment less than 1 month before start of the study and
during the study;
• Use of anti-depressives as a treatment for depression;
• Use of protein supplements (must be stopped 1 week before the first test
day);
• Reported weight loss or weight gain of > 3 kg in the month prior to pre-study
screening, or intention to lose weight during the study period;
• Reporting to follow or having planned a slimming or medically prescribed diet.
• Not willing to keep a stable lifestyle during the study period;
• Recent blood donation (<1 month prior to test day 1 of the study) or not
willing to stop donation during and 1 month after the study;
• Average alcohol intake >21 (women) or >28 (men) glasses of alcoholic
beverages per week;
• Use of drugs;
• Current smokers;
• Pregnant, lactating or wishing to become pregnant in the period of the study
(self-reported);
• Not having a general practitioner;
• Insufficient proficiency in Dutch to understand information brochure and
questionnaires;
• Participation in any clinical trial including blood sampling and/or
administration of substances up to 30 days before test day 1 of this study;
• Being an employee of the department Food, Health & Consumer Research of
Wageningen Food & Biobased Research or FrieslandCampina R&D.

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The main study parameter is the postprandial profile (iAUC and peak Cmax) of<br /><br>total essential amino acids (TEAA) before and after consumption of pea, faba,<br /><br>casein, and whey. </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Other study parameters include total and individual (essential) amino acid<br /><br>profiles (iAUC, peak Cmax, and Time-2-max) before and after consumption of pea,<br /><br>pea+methionine, pea+casein, faba, casein, and whey.</p><br>