Creation of a Bank of Fibroblast From Patients With Amyotrophic Lateral Sclerosis: Pilot Study
Not Applicable
Completed
- Conditions
- Amyotrophic Lateral Sclerosis
- Interventions
- Procedure: skin biopsy
- Registration Number
- NCT01639391
- Lead Sponsor
- Institut Pasteur
- Brief Summary
Patients with ALS will be included in the reference center for ALS in hospital La Pitié Salpêtrière, Paris.
The study proposes to investigate the pathophysiology of ALS by setting up a fibroblast bank for studying molecular, cellular and genetic parameters of the pathology.
iPS (induced pluripotent stem cells) and then differentiated cells will be generated.
The pathophysiology of ALS will be studied on the 3 types of cells (fibroblasts, iPS, differentiated cells).
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 19
Inclusion Criteria
- patients over 18 years old
- ALS sporadic or familial or unknown
Exclusion Criteria
- known cutaneous disease not allowing the biopsy
- platelets less than 10 000/m3
- suspected or known xylocain allergy
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- SINGLE_GROUP
- Arm && Interventions
Group Intervention Description patients skin biopsy -
- Primary Outcome Measures
Name Time Method Success of fibroblast culture, amplification, and freezing pellets 2 months
- Secondary Outcome Measures
Name Time Method Induced pluripotent Stem Cells generation success 2 years neurones, motoneurones, glial cells obtention from fibroblasts or iPS 2 years
Trial Locations
- Locations (1)
Centre référent maladies rares SLA
🇫🇷Paris, France
Centre référent maladies rares SLA🇫🇷Paris, France