Studies into the mechanism of measles-associated immune suppression during an outbreak of measles in The Netherlands
Completed
- Conditions
- Measles (Rubeola)10047438
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 200
Inclusion Criteria
In order to be eligible to participate in this study, a subject must meet all of the following criteria:
- Aged 4-17 years old
- Unvaccinated against measles
- No known history of natural measles
Exclusion Criteria
- Chronic disease
- Immune suppression (due to medication or underlying disease)
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Main study parameters/endpoints: The study consists of two cohorts. In cohort A<br /><br>clinical specimens (a blood sample and swabs of the upper respiratory tract)<br /><br>will be collected from children with an acute MV infection, shortly before, at<br /><br>or after onset of rash. The main study parameter in this group will be the<br /><br>characterization of MV-infected lymphocytes in peripheral blood, in order to<br /><br>confirm that the virus predominantly infects memory T- and B-lymphocytes. In<br /><br>cohort B blood samples will be collected from children that have not yet been<br /><br>exposed to MV. A second blood sample will be collected two to three weeks after<br /><br>recovery from measles. The main study parameter in this group will be the<br /><br>quantification of pre-existing memory lymphocyte populations in peripheral<br /><br>blood, to test the hypothesis that these cell populations are decimated during<br /><br>measles.</p><br>
- Secondary Outcome Measures
Name Time Method <p>As a secondary objective, we will try to validate and translate observations<br /><br>from our animal model studies to natural measles virus infections in children.<br /><br>To this end, we will measure hematological, virological and immunological<br /><br>parameters in white blood cells, plasma and swabs of the upper respiratory<br /><br>tract.</p><br>