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Biomarker Study of Immune-mediated Mechanism of Action of Neoadjuvant Trastuzumab in HER2+ Breast Cancer Patients

Phase 2
Conditions
Cancer, Breast
Interventions
Biological: Trastuzumab IV
Biological: Trastuzumab SC
Biological: Pertuzumab
Registration Number
NCT03144947
Lead Sponsor
Gruppo Oncologico Italiano di Ricerca Clinica
Brief Summary

Phase II, Open Label, Randomized, Biomarker Study of Immune-mediated Mechanism of Action of Neoadjuvant Subcutaneous (SC) Trastuzumab in Patients with Operable or Locally Advanced /Inflammatory HER2-positive Breast Cancer (ImmunHER)

Detailed Description

Women with histologically confirmed HER2-positive breast cancer with locally advanced, inflammatory,or early stage tumor (either greater than 2 cm in diameter or node positive) with no evidence of metastatic disease.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
65
Inclusion Criteria
  • Previously untreated, infiltrating primary breast cancer with locally advanced, inflammatory, or early stage tumor (either greater than 2 cm in diameter or node positive) with no evidence of metastatic disease.
  • HER2 positivity (either immunohistochemistry 3+ or fluorescent in situ hybridization amplification).
  • Age 18 or older.
  • Eastern Cooperative Oncology Group performance status of 0 to 1.
  • Availability of tumor tissue for biologic and molecular examination before starting primary treatment.
  • Left ventricular ejection fraction within the institutional range of normal.
  • Normal organ and marrow function.
  • Adequate contraception methods for women of childbearing potential.
  • Prior diagnosis of cancer is allowed as long as patient is free of disease and has been off treatment for the prior malignancy for a minimal interval of 3 years.
  • Written informed consent.
Exclusion Criteria
  • Either stage I or IV breast cancer.
  • Prior trastuzumab or pertuzumab.
  • Any prior chemotherapy.
  • Treatment with any other investigational agent, or participation in another clinical trial within 28 days prior to enrolment.
  • Undergone major surgery (e.g., intrathoracic, intra-abdominal or intra-pelvic) 4 weeks prior to starting study drug or who have not recovered from side effects of such surgery.
  • Breast radiotherapy prior to starting study.
  • Known hypersensitivity to the investigational drugs or any of their excipients.
  • Evidence of any disease, neurological or metabolic dysfunction, physical examination finding or laboratory finding giving reasonable suspicion of a disease or condition that contraindicates the use of an GOIRC-01-2016 ImmunHER Protocol Version 1.0, 11 April 2016 Page 6 of 140 investigational drug, or puts the patient at high risk for treatment-related complications.
  • Moderate/severe hepatic impairment (Child- Pugh B/C).
  • Uncontrolled inter-current illness including, but not limited to, ongoing or active infection, symptomatic congestive heart failure, unstable angina pectoris, cardiac arrhythmia, or psychiatric illness/social situations that would limit compliance with study requirements.
  • Concurrent malignancy or malignancy within 3 years prior to study enrollment, with the exception of adequately treated basal cell carcinoma, squamous cell carcinoma or other non-melanomatous skin cancer, or insitu carcinoma of the uterine cervix.
  • Pregnancy or breastfeeding (breast feeding should be discontinued to be enrolled in the study).
  • Women of childbearing potential that refusal to adopt adequate contraceptive measures.
  • Unwilling or unable to comply with the protocol. -

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Group ATrastuzumab IVTrastuzumab IV (8 mg/kg loading dose, followed by 6 mg/kg) plus pertuzumab IV (840 mg loading dose, followed by 420 mg) plus docetaxel (75 mg/m2)\*, every 3 weeks for 4 cycles. After surgery, study patients will receive trastuzumab IV x 14 cycles
Group BTrastuzumab SCTrastuzumab SC (fixed dose of 600 mg) plus pertuzumab IV (840 mg loading dose, followed by 420 mg) plus docetaxel (75 mg/m2)\*, every 3 weeks for 4 cycles. After surgery, study patients will receive trastuzumab SC x 14 cycles
Group BPertuzumabTrastuzumab SC (fixed dose of 600 mg) plus pertuzumab IV (840 mg loading dose, followed by 420 mg) plus docetaxel (75 mg/m2)\*, every 3 weeks for 4 cycles. After surgery, study patients will receive trastuzumab SC x 14 cycles
Group APertuzumabTrastuzumab IV (8 mg/kg loading dose, followed by 6 mg/kg) plus pertuzumab IV (840 mg loading dose, followed by 420 mg) plus docetaxel (75 mg/m2)\*, every 3 weeks for 4 cycles. After surgery, study patients will receive trastuzumab IV x 14 cycles
Group ADocetaxelTrastuzumab IV (8 mg/kg loading dose, followed by 6 mg/kg) plus pertuzumab IV (840 mg loading dose, followed by 420 mg) plus docetaxel (75 mg/m2)\*, every 3 weeks for 4 cycles. After surgery, study patients will receive trastuzumab IV x 14 cycles
Group BDocetaxelTrastuzumab SC (fixed dose of 600 mg) plus pertuzumab IV (840 mg loading dose, followed by 420 mg) plus docetaxel (75 mg/m2)\*, every 3 weeks for 4 cycles. After surgery, study patients will receive trastuzumab SC x 14 cycles
Primary Outcome Measures
NameTimeMethod
Tumor Infiltrating lymphocites (TIL) rate on residual disease after either IV trastuzumab or SC trastuzumab (see related paragraph)6 months after last patient in

stromal lymphocytes will be scored quantitatively on H\&E stained whole-tumor slides as a continuous variable expressed as stromal percentage area within the tumor boundaries. For tumors with heterogeneous TILs, median values will be calculated from multiple counts from different tumor areas. Intra-epithelial TILs will also be recorded as well as tertiary lymphoid structures. Tumor regression will be scored based on recommended criteria.

Secondary Outcome Measures
NameTimeMethod
HRQOL during study treatment based on FACT-Bat baseline, and 6 months after last patient in

mean FACT-B scores assessed at enrolment and mean FACT-B scores assessed before surgery.

Associations between biomarkers (TIL, Tumor specific lymphocyte cell activity (TLA), and Fc-gamma-R polymorphisms) and between each biomarker with clinical outcome variables.at baseline, 6 months and 5 years after last patient in
Complete pathological response rate by treatment arm6 months after last patient in
Frequency of toxicity Events: frequency of moderate and severe toxicity events and drop-out rate due to theraphy related toxicity (NCICommon Toxicity Criteria v 4.0)3.5 years
5-year disease-free survival by treatment arm between treatment arms5 years

Trial Locations

Locations (21)

UO di Oncologia Ematologia, Azienda Ospedaliero Universitaria di Ferrara

🇮🇹

Cona, Ferrara, Italy

Oncologia Medica, Ospedale Sacro Cuore - Don Calabria - Negrar (VR)

🇮🇹

Negrar, Verona, Italy

UOC di Oncologia. Azienda USL di Bologna, Ospedale Bellaria,

🇮🇹

Bologna, Italy

Oncologia Medica, IRST. Istituto Scientifico Romagnolo per lo studio e la cura dei Tumori, IRCCS di Meldola

🇮🇹

Meldola (FC), Italy

UO di Oncologia. Azienda USL di Rimini

🇮🇹

Rimini, Italy

Day Hospital, Ospedale di Sassuolo

🇮🇹

Sassuolo, Italy

U.O. di Oncologia Medica PO "S. Chiara"

🇮🇹

Trento, Italy

UOC Oncologia Medica, Azienda ULSS21 di Legnago

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Legnago, Verona, Italy

Breast Unit Spedali Civili di Brescia

🇮🇹

Brescia, Italy

Investigational Clinical Oncology - INCOIRCCS-Fondazione del Piemonte per l'Oncologia (FPO)

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Candiolo, Italy

Chirurgia generale ad indirizzo senologico-Breast Unit Azienda Istituti Ospitalieri di Cremona

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Cremona, Italy

Dipartimento di Medicina Interna e Specialità Mediche (DI.M.I.)-Università di Genova Clinica di Medicina Interna ad indirizzo oncologico

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Genova, Italy

UOC Oncologia-A.O. PAPA GIOVANNI XXIII Bergamo

🇮🇹

Bergamo, Italy

SSD di Oncologia Medica Addarii, Policlinico S. Orsola-Malpighi,

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Bologna, Italy

Divisione di Oncologia Medica - Ospedale di Bolzano,

🇮🇹

Bolzano, Italy

Dipartimento di Scienze Mediche e Chirurgiche, Materno Infantili e dell'adulto. Policlinico di Modena

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Modena, Italy

SC di Oncologia Medica, A.O. San Gerardo

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Monza, Italy

Azienda Ospedaliero-Universitaria di Parma, UOC di Oncologia Medica

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Parma, Italy

Dipartimento di Oncologia e Ematologia, UO di Oncologia Medica Azienda USL di Piacenza

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Piacenza, Italy

Struttura Complessa di OncologiaIRCCS- Istituto in Tecnologie Avanzate e Modelli Assistenziali in Oncologia Arcispedale Santa Maria Nuova

🇮🇹

Reggio Emilia, Italy

Oncologia Medica Az. Ospedaliera di Verona

🇮🇹

Verona, Italy

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