Functional Characterization of CD8+ T Cells After Supramaximal Exercise
- Conditions
- Exercise Induced CD8+ T Cell Recruitment and Characterization of Function and Phenotype of These Cells
- Interventions
- Procedure: Exercise
- Registration Number
- NCT06484101
- Lead Sponsor
- Karolinska Institutet
- Brief Summary
Healthy human subjects perform supramaximal exercise with venous blood samples obtained before and up to 60 minutes after the exercise bout.
- Detailed Description
Healthy, human subjects perform 3 x 30 seconds of supramaximal exercise. Venous blood samples were obtained before and up to 60 minutes after the exercise bout. The blood was used for absolute count of lymphocytes and monocytes per volume blood, and for isolation of CD8+ T cells. It was also used for blood gas analysis. The CD8+ T cells were characterized using flow cytometry, and then cultured for 7 days with CD8+ activation beads. The cells were counted and analyzed for viability at day4 and day8. RNAseq was performed on CD8+ cells isolated before and directly after exercise. CD8+ T cells from Day 1 pre and post exercise and then after 7 days of culture, were analyzed for IFNgamma release using Elispot, and for cytotoxicity against HEK cancer cells in vitro. Flow cytometry of CD8+ T cells was performed on fresh CD8 T cells pre and post exercise, and then on day4 and day8 after culture. SeaHorse metabolic assay was done on Day1 CD8 T cells obtained pre and post exercise.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 7
- Be used to physical exercise and be able to perform strenous exercise Be able to attend the experimental procedure for the 90 minute duration of the study
- Smoking Pregnant Have any medical condition requiring medical treatment
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- SINGLE_GROUP
- Arm && Interventions
Group Intervention Description Exercise Exercise Healthy human subjects performing 3 x 30 seconds of supramaximal exercise with blood obtained before and up to 60 minutes after the exercise bout
- Primary Outcome Measures
Name Time Method CD8 T cell pheontype after exercise Before, directly after, 30 minutes and 60 minutes after exercise Flow cytometry analysis of CD8 T cells
CD8 T cell absolute nr after exercise Before, directly after, 60 minutes after exercise CD8 nr
CD8 T cell cytotoxicity against cancer cells Cryopreserved CD8 cells from Day 1 and Day 8 of the experiment, obtained before and after exercise CD8 T cell lysis of cancer cells
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Karolinska Sjukhuset Huddinge
🇸🇪Huddinge, Stockholm, Sweden