Major Allergen in Wheat Anaphylaxis in Thai Population

Not Applicable

Completed

• Conditions: Wheat Allergy; Anaphylaxis Food; Anaphylaxis Exercise
• Interventions: Diagnostic Test: immunoblot

• Registration Number: NCT03487315
• Lead Sponsor: Mahidol University

Brief Summary

* To study Major allergen in Wheat anaphylaxis in Thai population

* To study and compare demographic data between group of wheat anaphylaxis

Detailed Description

Wheat is one of the most common causes in food-induced anaphylaxis and food dependent, exercised induced anaphylaxis. Gold standard for diagnose food allergy is double blind placebo control food challenge, but it is a high risk procedure. The accuracy of skin prick test and specific IgE assay showed unsatisfactory results. It might be explained by these tests may not detect all of the major allergens in wheat allergic patients. Previous studies showed that the major allergens of wheat dependent, exercised induced anaphylaxis are omega5 gliadin, followed by high molecular weight glutenin. For IgE-mediated wheat allergy, the major allergens varied among studies, including omega5 gliadin, glutenin, alpha/beta/gamma gliadin, and the water-soluble part of wheat allergens. There is no study to direct compare the major allergen between patients who have low wheat specific IgE and very high wheat specific IgE. Also, there is no data about the major allergens of wheat anaphylaxis in Thai population.

Basic Information
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Recruitment & Eligibility
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Study & Design
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Trial Locations

Study Timeline

• Study Start: 2017-04-05
• Study First Submitted: 2017-08-31
• Status Verified: 2018-03
• Primary Completion: 2018-03-05
• Study Completion: 2018-03-05
• Study First Submitted QC: 2018-03-27
• Last Update Submitted: 2018-03-27
• Study First Posted: 2018-04-04
• Last Update Posted: 2018-04-04

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 36

Inclusion Criteria

• Wheat anaphylaxis patient aged 1-60 years, who received treatment at division of allergy, department of Pediatrics and Medicine, Siriraj Hospital from 2001 to 2016

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Exclusion Criteria

• Loss follow up
• No consent form
• Celiac disease, Autoimmune disease was diagnosed

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
specific IgE	immunoblot	Different level of specific IgE and immunoblot

Primary Outcome Measures

Name	Time	Method
Major allergen sensitized by Thai Patients with Wheat anaphylaxis	1 hour	To Identify major allergen (most component of protein that cause wheat allergy) between wheat anaphylaxis (low Specific IgE and high Specific IgE) and wheat dependent, exercise induced anaphylaxis by using immunoblot method (Electrophoresis). The results will be shown in electrophoresis gel and can identify in molecular weight (KDa) of protein which represent to major allergen of wheat protein.

Trial Locations

Locations (1)

Siriraj Hospital; 🇹🇭 Bangkok Noi, Bangkok, Thailand