Analysis of the acetabular chondral flap (ALAD-3/4 damage) in FAI syndrome type CAM
Not Applicable
Recruiting
- Conditions
- M24.85M94.25
- Registration Number
- DRKS00023506
- Lead Sponsor
- Klinik und Poliklinik für Orthopädie und orthopädische ChirurgieForschungsabteilung | Human Cells and Orthopedic MaterialsUniversitätsmedizin Greifswald KöR
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- All
- Target Recruitment
- 10
Inclusion Criteria
FAI Syndrome, chondral damage ALAD 3/4
Exclusion Criteria
Chondral damage ALAD 1/2
Study & Design
- Study Type
- interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method nstable chondral delamination flaps are routinely resected intraoperatively and examined. <br>Histology: A portion of the resected tissue is fixed in paraformaldehyde for histological examination, embedded in kerosene and cut. For histological evaluation of cartilage quality, the following staining procedures are performed: (i) H&E as overview staining and for the evaluation of possible immune cell infiltrations (ii) Collagen (II) Immunofluorescence for the evaluation of cartilage quality or the proportion of hyaline articular cartilage (iii) Alcian blue for the evaluation of cartilage quality or the proportion of sulfated glycosaminoglycans. <br>
- Secondary Outcome Measures
Name Time Method Flow cytometry: A part of the resectate is digested for cell isolation by collagenase degradation (Yonenaga et al. 2017). After degradation the cell counts / g resectate are quantified. To assess possible immune cell infiltration, the isolated cells are examined by flow cytometry. For this purpose a Basic Immune Phenotyping (CD3, CD4, CD8, CDM, CD16, CD19, CD45, CD56) is performed. <br><br>Cell characterization: To assess the functionality of isolated chondrocytes, they are expanded in vitro and subsequently characterized, (i) proliferation capacity and viability, (ii) migration capacity (live cell imaging), (iii) matrix formation after chondrogenic in vitro stimulation and subsequent proteoglycan quantification, histological examination of the cartilage pellet and gene expression analyses of the classical chondrogenic markers (ACAN, MMP13, SOX9, COL10A1, COL2A1) before and after stimulation.<br>