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Oral Contraceptive Consumption Timing and Cognition and Metabolism

Not Applicable
Completed
Conditions
Cognitive Change
Interventions
Drug: Combined oral contraceptive pill
Registration Number
NCT06560944
Lead Sponsor
University of Lincoln
Brief Summary

The aim of this randomised-controlled trial is to examine whether the timing of oral contraceptive administration affects cognitive function, substrate metabolism and exercise performance.

Participants will attend the laboratory for a familiarisation session, followed by two main trials in which cognition and exercise metabolism and performance are measured. In one trial they will be given their oral contraceptive 60 minutes before testing begins, in the other they will be given a placebo. Following testing (\~60 minutes), participants will consume the alternative pill.

Detailed Description

Reproductive hormones, such as oestrogen and progesterone, are known to affect cognitive function (i.e. thinking, learning and processing information) and substrate metabolism (i.e. how the body uses carbohydrates and fats). Oral contraceptives contain synthetic versions of reproductive hormones and are used by approximately half of women of reproductive age. When oral contraceptives are ingested, the concentrations of these synthetic hormones spike in the blood for several hours, yet no research has explored the effect of this spike on how the body works, especially with regards to cognitive function and substrate metabolism. If this spike in synthetic hormone concentrations affects cognition or metabolism, this could influence the way in which women use oral contraceptives, such as altering the timing of ingestion to improve performance and health outcomes.

The proposed study will use participants that currently use a specific preparation of oral contraceptive (30micrograms ethinyl estradiol and 150mg Levonorgestrel) and compare cognitive function and substrate metabolism on an occasion when a placebo is consumed (lactose - no hormones), to an occasion when their oral contraceptive is consumed. It takes approximately 60-90 minutes for concentrations of hormones to peak in the blood following ingestion, therefore measurements of cognitive function and substrate metabolism will be taken at this time. Cognitive function will be measured using a battery of tests administered orally or via a laptop and substrate metabolism will be measured by collecting expired air via a mouthpiece at rest and during exercise on a treadmill, during which time perceived difficulty of the exercise (rate of perceived exertion) and feelings (felt arousal) will be measured. Venous blood samples will be drawn at baseline (immediately before consumption of first pill/placebo) and 90 minutes following this. The participants will consume either the placebo or oral contraceptive (whichever was not initially consumed) following the testing protocol to ensure that their contraceptive protection is maintained.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
16
Inclusion Criteria
  • Female
  • Aged 18-35 years
  • The use of the same oral contraceptive (30 micrograms ethinyl estradiol and 150mg levonorgestrel) for at least 6 months
  • Physically active and able to take part in treadmill-based exercise
  • Speak fluent English
Exclusion Criteria
  • Use of any other type of hormonal contraception
  • Aged < 18 or > 35 years
  • Poly-cystic ovarian syndrome
  • Endometriosis
  • Pregnant
  • Child birth or lactation in the previous 6 months
  • Body mass index < 18.5 or > 30 kg.m2
  • Any disorder known to affect metabolic health
  • History of head injury/neurological disorders
  • Use of any steroid-based medication or medications known to influence the central nervous system
  • History of psychiatric disorders such as major depression or an anxiety disorder
  • Smokers
  • Suffer from (or have suffered from) a heart complaint
  • Advised by medical professional to refrain from high intensity exercise
  • Current muscle or joint injury

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Oral contraceptiveCombined oral contraceptive pillParticipants existing oral contraceptive to be ingested at 8am on a day between 3 to 21 of pill consumption. Placebo consumed \~2 hours later
PlaceboCombined oral contraceptive pillPlacebo ingested at 8am on a day between 3 to 21 of pill consumption. Participants existing oral contraceptive consumed \~2 hours later
Primary Outcome Measures
NameTimeMethod
Rey Auditory Verbal Test Performance60-90 minutes post oral contraceptive/placebo ingestion

Acquisition, Learning rate, Proactive Interference, Retroactive interference, Forgetting

Mental Rotation test60-90 minutes post oral contraceptive/placebo ingestion

Accuracy, Response time

Verbal Fluency60-90 minutes post oral contraceptive/placebo ingestion

Words said

Stroop test60-90 minutes post oral contraceptive/placebo ingestion

Accuracy, Response time

Rapid Visual Information Processing60-90 minutes post oral contraceptive/placebo ingestion

Proportion correct, Response time

Corsi blocks60-90 minutes post oral contraceptive/placebo ingestion

Correct sequences, longest sequence

Feeling scaleDuring final minute of of 6 minutes exercise at both 70 and 90% lactate threshold. Exercise takes place approximately 90 minutes post oral contraceptive/placebo ingestion

Feeling scale -5 to +5. Higher value indicates more positive feeling.

Heart rateDuring final minute of of 6 minutes exercise at both 70 and 90% lactate threshold. Exercise takes place approximately 90 minutes post oral contraceptive/placebo ingestion

beats per minute

Rating of perceived exertionDuring final minute of of 6 minutes exercise at both 70 and 90% lactate threshold. Exercise takes place approximately 90 minutes post oral contraceptive/placebo ingestion

Borg rating of perceived exertion (RPE) scale from 6-20. Higher value indicates greater exertion

Felt arousalDuring final minute of of 6 minutes exercise at both 70 and 90% lactate threshold. Exercise takes place approximately 90 minutes post oral contraceptive/placebo ingestion

Felt arousal scale from 1-6. Higher value indicates greater level of arousal

Maximal oxygen consumption (VO2max)During maximal exercise test which occurs approximately 110 minutes post oral contraceptive/placebo ingestion

Maximal rate of oxygen used

Maximal heart rateDuring maximal exercise test which occurs approximately 110 minutes post oral contraceptive/placebo ingestion

Maximal heart rate achieved

Carbohydrate oxidationDuring final minute of of 6 minutes exercise at both 70 and 90% lactate threshold. Exercise takes place approximately 90 minutes post oral contraceptive/placebo ingestion

Rate of carbohydrate use

Respiratory exchange ratioDuring final minute of of 6 minutes exercise at both 70 and 90% lactate threshold. Exercise takes place approximately 90 minutes post oral contraceptive/placebo ingestion

Ratio of carbohydrate and fat metabolism

Fat oxidationDuring final minute of of 6 minutes exercise at both 70 and 90% lactate threshold. Exercise takes place approximately 90 minutes post oral contraceptive/placebo ingestion

Rate of fat use

Breathing frequencyDuring final minute of of 6 minutes exercise at both 70 and 90% lactate threshold. Exercise takes place approximately 90 minutes post oral contraceptive/placebo ingestion

breaths per minute

Time to exhaustionDuring maximal exercise test which occurs approximately 110 minutes post oral contraceptive/placebo ingestion

Time before test termination

Secondary Outcome Measures
NameTimeMethod
Seum ethinyl estradiolBaseline and immediately post cognitive function tests

Synthetic oestrogen concentrations in blood

Serum LevonorgestrelBaseline and immediately post cognitive function tests

Synthetic progesterone concentrations in blood

Trial Locations

Locations (1)

Dan Martin

🇬🇧

Lincoln, Lincolnshire, United Kingdom

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