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Clinical Trials/NCT01173705
NCT01173705TerminatedNot Applicable

Genetic and Nutritional Factors Involved in Obesity-related Inflammation and Insulin Resistance

Vanderbilt University1 site in 1 country62 target enrollmentStarted: June 2011Last updated:
Conditions

Trial Snapshot

Phase
Not Applicable
Status
Terminated
Enrollment
62
Locations
1
Primary Endpoint
Examine the relationship between omega-3 content and inflammatory and oxidative stress markers

Study Overview

Brief Summary

In this project, we propose to recruit lean and obese subjects with different ethnic background (African Americans and Caucasians) to study the alterations of lipid and carbohydrate metabolism and determine whether these disturbances are linked to genetic, inflammatory, oxidative stress, and/or nutritional factors. Because systemic inflammation and insulin resistance are frequent features of obesity, we postulate that an unbalanced diet with high saturated- and low omega 3-fatty acids is linked to obesity-related inflammation and insulin resistance. We propose to investigate fatty acid metabolism and determine the links between fatty acid composition and oxidative stress in tissues of lean and obese subjects. We propose the following aims:

Specific Aim 1: Evaluate nutrient intake in lean and obese subjects using the standard NHANES Food Questionnaire.

Specific Aim 2: Evaluate the fatty acid composition, including omega-3, in adipose tissue depots, blood monocytes and skeletal muscle, and examine the relationship between omega-3 content and inflammatory and oxidative stress markers.

Specific Aim 3: Compare the effects of omega-3 and saturated FA supplementation on inflammatory and oxidative stress markers in vitro in adipose tissue explants, preadipocytes and monocyte culture.

Study Design

Study Type
Observational
Observational Model
Case Control
Time Perspective
Cross Sectional

Eligibility Criteria

Ages
18 Years to 65 Years (Adult, Older Adult)
Sex
All
Accepts Healthy Volunteers
No

Inclusion Criteria

  • 18-65 years of age
  • Undergoing elective abdominal or bariatric surgery

Exclusion Criteria

  • Intercurrent infections
  • Active cancer diagnosis within 5 years
  • Use of medications for diabetes or hyperlipidemia
  • Use of glucocorticoids or anti-inflammatory drugs

Outcomes

Primary Outcomes

Examine the relationship between omega-3 content and inflammatory and oxidative stress markers

Time Frame: 5 years

We will evaluate fatty acid composition in adipose tissues depots, blood monocytes, and skeletal muscle to examine the relationship between omega-e content and inflammatory and oxidative stress markers.

Secondary Outcomes

  • Evaluate nutrient intake(5 years)
  • Compare effects of omega-3 and saturated fatty acid supplementation on inflammatory and oxidative stress markers (in vitro)(5 years)

Investigators

Sponsor Class
Other
Responsible Party
Principal Investigator
Principal Investigator

Robyn Tamboli

Research Assistant Professor

Vanderbilt University

Study Sites (1)

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