Genetic and Nutritional Factors Involved in Obesity-related Inflammation and Insulin Resistance
Trial Snapshot
- Phase
- Not Applicable
- Status
- Terminated
- Sponsor
- Vanderbilt University
- Enrollment
- 62
- Locations
- 1
- Primary Endpoint
- Examine the relationship between omega-3 content and inflammatory and oxidative stress markers
Study Overview
Brief Summary
In this project, we propose to recruit lean and obese subjects with different ethnic background (African Americans and Caucasians) to study the alterations of lipid and carbohydrate metabolism and determine whether these disturbances are linked to genetic, inflammatory, oxidative stress, and/or nutritional factors. Because systemic inflammation and insulin resistance are frequent features of obesity, we postulate that an unbalanced diet with high saturated- and low omega 3-fatty acids is linked to obesity-related inflammation and insulin resistance. We propose to investigate fatty acid metabolism and determine the links between fatty acid composition and oxidative stress in tissues of lean and obese subjects. We propose the following aims:
Specific Aim 1: Evaluate nutrient intake in lean and obese subjects using the standard NHANES Food Questionnaire.
Specific Aim 2: Evaluate the fatty acid composition, including omega-3, in adipose tissue depots, blood monocytes and skeletal muscle, and examine the relationship between omega-3 content and inflammatory and oxidative stress markers.
Specific Aim 3: Compare the effects of omega-3 and saturated FA supplementation on inflammatory and oxidative stress markers in vitro in adipose tissue explants, preadipocytes and monocyte culture.
Study Design
- Study Type
- Observational
- Observational Model
- Case Control
- Time Perspective
- Cross Sectional
Eligibility Criteria
- Ages
- 18 Years to 65 Years (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •18-65 years of age
- •Undergoing elective abdominal or bariatric surgery
Exclusion Criteria
- •Intercurrent infections
- •Active cancer diagnosis within 5 years
- •Use of medications for diabetes or hyperlipidemia
- •Use of glucocorticoids or anti-inflammatory drugs
Outcomes
Primary Outcomes
Examine the relationship between omega-3 content and inflammatory and oxidative stress markers
Time Frame: 5 years
We will evaluate fatty acid composition in adipose tissues depots, blood monocytes, and skeletal muscle to examine the relationship between omega-e content and inflammatory and oxidative stress markers.
Secondary Outcomes
- Evaluate nutrient intake(5 years)
- Compare effects of omega-3 and saturated fatty acid supplementation on inflammatory and oxidative stress markers (in vitro)(5 years)
Investigators
Robyn Tamboli
Research Assistant Professor
Vanderbilt University