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Acute Lung Injury After Cardiac Surgery: Pathogenesis

Completed
Conditions
Inflammation
Coagulation; Intravascular
Postoperative; Dysfunction Following Cardiac Surgery
Acute Lung Injury
Metabolic Disturbances
Registration Number
NCT02475694
Lead Sponsor
Aalborg University Hospital
Brief Summary

The purpose of the present study is to visualize the inflammatory response and coagulation disorders during cardiac surgery in order to identify possible predictors for acute lung injury postoperatively.

Detailed Description

Acute lung injury after cardiac surgery is a common complication. The pathophysiology is complex and still not fully elucidate. No specific biomarkers are identified and the diagnosis is solely based on impaired oxygenation.

The purpose of the present study is to identify biomarkers of inflammation and coagulation in blood withdrawn simultaneous from the pulmonary artery and the left atrium during surgery and up to 20 hours after weaning from cardiopulmonary bypass.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
50
Inclusion Criteria
  • Elective surgery
  • CABG
  • In treatment with statins
Exclusion Criteria
  • Acute surgery
  • Valve replacement
  • In treatment with immunodepressive drugs

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Acute lung injury measured as the fraction of partial pressure of oxygen divided by the fraction of inspired oxygenEvaluated 72 hours after weaning from cardiopulmonary bypass (CPB)

Arterial blood gas analysis

Secondary Outcome Measures
NameTimeMethod
Interleukin-1betaThe day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB

Measured in blood samples from radial artery, pulmonary artery and left atrium

D-dimerThe day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB

Measured in blood samples from pulmonary artery and left atrium

Interleukin-2The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB

Measured in blood samples from radial artery, pulmonary artery and left atrium

Interleukin-4The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB

Measured in blood samples from radial artery, pulmonary artery and left atrium

Interleukin-6The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB

Measured in blood samples from radial artery, pulmonary artery and left atrium

Interleukin-8The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB

Measured in blood samples from radial artery, pulmonary artery and left atrium

Interleukin-10The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB

Measured in blood samples from radial artery, pulmonary artery and left atrium

Tumor necroting factor-alphaThe day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB

Measured in blood samples from radial artery, pulmonary artery and left atrium

Thrombin-antithrombin complexesThe day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB

Measured in blood samples from pulmonary artery and left atrium

Plasmin-antiplasmin complexesThe day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB

Measured in blood samples from pulmonary artery and left atrium

Interferon-gammaThe day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB

Measured in blood samples from radial artery, pulmonary artery and left atrium

Granulocyte-macrophage colony-stimulating factorThe day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB

Measured in blood samples from radial artery, pulmonary artery and left atrium

Thrombin Fragment 1.2The day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB

Measured in blood samples from pulmonary artery and left atrium

MetabolomicsThe day before surgery; the day of surgery with measurement just before connecting to , immediately after weaning from CPB, 2, 4, 8, 16 and 20 hours after weaning from CPB; and exactly 48 and 72 hours after weaning from CPB

Metabolomics using the NMR-technique in blood samples from radial artery, pulmonary artery and left atrium

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