Can the Origin of Sperm - Ejaculated or Testicular- Affect Embryo Ploidy in Patients With Severe Male Factor Infertility?
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 100
- 试验地点
- 2
- 主要终点
- Euploidy rate
研究概览
简要总结
There is evidence indicating that sperm plays a role in determining blastocyst ploidy status. In our study, we aim to compare the outcomes of preimplantation genetic testing for aneuploidy (PGT-A) and the subsequent clinical outcomes following transfers of euploid embryos. This comparison will be conducted between cases where ejaculated sperm and cases where testicular sperm were used for intracytoplasmic sperm injection (ICSI), specifically focusing on severe male factor infertility patients.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 50 Years（Child, Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Cases planned to have ICSI PGT-A cycles
- •Males diagnosed as severe male factor infertility
- •Normo responder female partners
排除标准
- •Leukocytospermia
- •Presence of varicocele.
- •Known genetic abnormality
- •Use of sperm or oocyte donors
- •Use of gestational carrier
- •Presence of any of the endometrial factors that affect embryo implantation such as hydrosalpings, adenomyosis or previous uterine infection
- •Any contradictions to undergoing in vitro fertilization or gonadotropin stimulation
结局指标
主要结局
Euploidy rate
时间窗: 15 days post ICSI
Defined as the proportion of euploid blastocysts
Aneuploidy rate
时间窗: 15 days post ICSI
Defined as the proportion of aneuploid blastocysts
Low mosaic rate
时间窗: Time Frame: 15 days post ICSI
Defined as the proportion of low mosaic blastocysts
High mosaic rate
时间窗: Time Frame: 15 days post ICSI
Defined as the proportion of high mosaic blastocysts
Implantation rate
时间窗: 6-9 weeks post-embryo transfer
defined as the percentage of successfully implanted embryos
Ongoing pregnancy rate
时间窗: 12 weeks of gestation
defined as a positive heartbeat on ultrasound beyond 12 weeks of gestation
Miscarriage rate
时间窗: after 6 weeks of gestation
defined as pregnancy loss following confirmation of clinical pregnancy
Clinical pregnancy rate
时间窗: 6-9 weeks post-embryo transfer
defined as the presence of a fetal heartbeat or gestational sac 6-9 weeks post-embryo transfer
次要结局
- Fertilization rate(1 day)
- Cleavage rate(3 days)
- Blastocyst development rate(5-6 days)