Comparative Microbial Epidemiology and Chlorhexidine Suscebtibily of Oropharyngeal and Intestinal Colonization of Healthy Adults Subjects
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Enrollment
- 100
- Locations
- 1
- Primary Endpoint
- proportion of patients with an oropharyngeal gram-negative colonization
Study Overview
Brief Summary
In this prospective observational study we aim to determine the prevalence of oropharyngeal and rectal Gram-negative bacilli colonization in healthy subjects, and their susceptibility to chlorehexidine. We plan to recruit 100 healthy volunteers secondary endpoints are to determine the phylogentic characteristics of E. coli isolates; to compare the phylogentic characteristics of oropharyngeal and rectal of predominant GNB colonization.
Detailed Description
In this prospective observational study we aim to determine the prevalence of oropharyngeal and rectal Gram-negative bacilli colonization in 100 healthy subjects, and their susceptibility to chlorehexidine. Each participant will undego a oropharyngeal swab collection and a rectal swab at day 0. The Gram-negative bacilli colonization will be identified, E. coli isolates will be studied in terms of phylogeny; gram-negative isolates will be studied in terms of antimicrobial and chlorhexidine susceptibility. The primary objective is to determine the prevalence of oropharyngeal Gram-negative bacilli colonization. The secondary objectives are to determine the chlorhexidine and antimicrobial susceptibility of such colonization; to determine the phylogentic characteristics of oropharyngeal and rectal predominant E. coli isolates; to compare the phylogentic characteristics of oropharyngeal and rectal E. coli isolates.
Study Design
- Study Type
- Observational
- Observational Model
- Ecologic Or Community
- Time Perspective
- Prospective
Eligibility Criteria
- Ages
- 18 Years to — (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •age > 18 yo
- •personal history of neck or face irradiation
- •no history of gastrointestinal disease
- •no symptoms of immunosuppression
- •no antibiotic therapy in the previous month
- •no hospitalization in the 3 months preceding inclusion
- •medical exmination prior to inclusion
Exclusion Criteria
- •affiliated to the social security
- •The refusal of a patient to participate in a study
Outcomes
Primary Outcomes
proportion of patients with an oropharyngeal gram-negative colonization
Time Frame: Day 1
Secondary Outcomes
- characterization of antimicrobial and chlorhexidine susceptibility of Gram negative bacteria from oropharyngeal colonization(Day 1)
- comparision of the predominant fecal flora with GNB oropharyngeal colonization(Day 1)
- phylogenetic and virulence factor content of E. coli oropharyngeal and rectal colonization(Day 1)
- incidence of the a GNB oropharyngeal colonization(Day 1)