Role of endometrial injury in the success of IVF in patients with failed implantation in previous IVF cycles and its genetic basis.

Not Applicable

Conditions: Health Condition 1: null- healthy and with failed implantation in previous IVF cycle.

Recruitment & Eligibility

Inclusion Criteria

1.Women less than 38 years old.

2.Women who have >1 previous failed IVF-ET cycles.

3.Women with good ovarian reserve (AFC >8, AMH 2-6 ng/ml, FSH level <8 mIU/ml)

4.No uterine manipulation within last 3 months (e.g. hysteroscopy, myomectomy etc)

5.Women willing to participate in the trial.

Exclusion Criteria

1.Women more than 38 years old with confounding factors(eg Poor ovarian reserve)

2.Women with Grade III & IV Endometriosis.

3.Women with history of septal resection, adhesiolysis &abnormal uterine cavity.

4.Women with possible causes for failure of implantation such as diabetes mellitus, hypertension, autoimmune diseases..

5.Women not willing to participate in the trial.

Primary Outcome Measures

Name	Time	Method
1) Implantation rate (Time frame; 15-20 days of embryo transfer, serum <br/ ><br>beta hCG level) <br/ ><br> Implantation rate will be calculated as <br/ ><br>a) Number of patients with positive Serum beta hCG on Day 16 of ET <br/ ><br>b) Number of gestational sacs as seen on transvaginal sonography divided by the number of embryos transferred. <br/ ><br>2) Clinical pregnancy rate (Time frame; 8-10 weeks, appearance of <br/ ><br>cardiac activity) <br/ ><br>3) Genomic wide Transcriptomic basis <br/ ><br>Timepoint: 1) Implantation rate (Time frame; 15-20 days of embryo transfer, serum <br/ ><br>beta hCG level) <br/ ><br> Implantation rate will be calculated as <br/ ><br>a) Number of patients with positive Serum beta hCG on Day 16 of ET <br/ ><br>b) Number of gestational sacs as seen on transvaginal sonography divided by the number of embryos transferred. <br/ ><br>2) Clinical pregnancy rate (Time frame; 8-10 weeks, appearance of <br/ ><br>cardiac activity) <br/ ><br>3) Genomic wide Transcriptomic basis

Secondary Outcome Measures

Name	Time	Method
1. Pregnancy rate/ ongoing pregnancy rate. (Time frame; till 12 weeks of <br/ ><br>gestation) <br/ ><br>2. Miscarriage Rate (Time frame: till 12 weeks of gestation) <br/ ><br>3. Endometrial thickness during stimulation (Time frame: during <br/ ><br>stimulation cycle designated as safety issue: No <br/ ><br>Timepoint: 1. Pregnancy rate/ ongoing pregnancy rate. (Time frame; till 12 weeks of <br/ ><br>gestation) <br/ ><br>2. Miscarriage Rate (Time frame: till 12 weeks of gestation) <br/ ><br>3. Endometrial thickness during stimulation (Time frame: during <br/ ><br>stimulation cycle designated as safety issue: No