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Evaluation of Kisspeptin Glucose-Stimulated Insulin Secretion With Oral Glucose Tolerance Test

Phase 1
Completed
Conditions
Metabolic Diseases
Interventions
Diagnostic Test: Oral Glucose Tolerance Test
Drug: Placebo
Registration Number
NCT04958109
Lead Sponsor
Massachusetts General Hospital
Brief Summary

The goal of this study is to understand how exogenous kisspeptin affects metabolism by evaluating responses to an oral glucose tolerance test

Detailed Description

Assignment: Each study subject will serve as their own control. The order of the visits will be randomized.

Delivery of Interventions:

* Prior to the study visit, subjects will undergo a review of their medical history and screening laboratories.

* During the inpatient study, the subjects will

* Undergo a 16-hour kisspeptin infusion

* Undergo an oral glucose tolerance test

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
16
Inclusion Criteria

History:

  • over the age of 18,
  • normal pubertal development
  • stable weight for previous three months,
  • normal body mass index (BMI between 18.5-25)
  • regular menstrual cycles

Physical examination:

• systolic BP < 140 mm Hg, diastolic < 90 mm Hg

Laboratory studies: (per Massachusetts General Hospital reference ranges)

  • normal hemoglobin
  • hemoglobin A1C < 6.5%
  • blood urea nitrogen, creatinine not elevated
  • aspartate aminotransferase, alanine aminotransferase < 3x upper limit of normal
Exclusion Criteria
  • active illicit drug use,
  • history of a medication reaction requiring emergency medical care,
  • difficulty with blood draws.
  • history of chronic disease, except well controlled thyroid disease,
  • recent use of prescription medications which interfere with metabolism or reproduction (recent = within 5 half-lives of the drug). Use of levothyroxine or seasonal allergy medications is acceptable,
  • history of diabetes in a first degree relative,
  • use of contraceptive pills, patches or vaginal rings within last 4 weeks.
  • hyperlipidemia by fasting lipid panel
  • positive serum pregnancy test (for all women)

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
KisspeptinOral Glucose Tolerance Test• Intravenous administration of kisspeptin 112-121 x 16 hours
PlaceboPlacebo• Intravenous administration of placebo x 16 hours
KisspeptinKisspeptin• Intravenous administration of kisspeptin 112-121 x 16 hours
PlaceboOral Glucose Tolerance Test• Intravenous administration of placebo x 16 hours
Primary Outcome Measures
NameTimeMethod
Oral Glucose Sensitivity Index (Kisspeptin-Placebo)3 hours

Change in Oral glucose Sensitivity Index between kisspeptin and placebo arms in oral glucose tolerance test

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Massachusetts General Hospital

🇺🇸

Boston, Massachusetts, United States

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