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Modulation of the Brain Excitatory/Inhibitory (E/I) Balance in Autism Spectrum Disorder (ASD)

Not Applicable
Completed
Conditions
Autism Spectrum Disorder
Interventions
Drug: Arbaclofen_15
Drug: Arbaclofen_30
Drug: Placebo
Registration Number
NCT03594552
Lead Sponsor
King's College London
Brief Summary

This study investigates the brain response to a single acute dose of Arbaclofen, the R-enantiomer of the GABA-B agonist Baclofen, compared to a single dose of placebo in healthy men with and without autism spectrum disorder.

Detailed Description

Previous research suggests that GABAergic drug compounds could shift brain excitation and inhibition (E-I) in the healthy brain and in neurodevelopmental psychiatric conditions, such as autism spectrum disorder (ASD) - where this balance is disrupted. A study by Ajram et al. (2017) has shown an E-I shifted towards more GABA in individuals with ASD, and not in controls, after a single dose of the anti-glutamatergic and pro-GABAergic drug Riluzole. Moreover, brain connectivity patterns in ASD patients where shifted towards the ones observed in the control group. However, it was unclear whether this changes could be driven by GABA receptors, thus more specific probes may help to clarify the mechanism underlying the E-I coordination in ASD. Therefore, this study will use neuroimaging and electrophysiology to investigate the brain E-I coordination in ASD compared to control participants when the system is responding to a single dose of the specific GABA-B (STX209) receptor agonist. 50 adult individuals with ASD and 50 neurotypical adults (25 males and 25 females per group) will be invited to participate. Each participant will receive a single dose of the drug (15mg or 30mg Arbaclofen) or matched placebo). Brain activity and neurochemistry will be investigated using magnetic resonance imaging. Further data will be collected through questionnaires, behavioural tasks, blood samples, and sensory tasks using electroencephalography and retinal imaging.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
87
Inclusion Criteria
  • ASD participants must pass diagnostic threshold for ASD on the Autism Diagnostic Interview-Revised (if and informant is available)
  • ASD participants must be currently symptomatic on Autism Diagnostic Observation Schedule (ADOS)
  • Age 18-60 years
  • Can give informed consent
  • medication free in the month preceding participation; but regular medication (used in a stable dose over the two months previous to participation) with drug which does not affect glutamate or GABA directly may be permitted
  • IQ>70
Exclusion Criteria
  • IQ<70
  • history of psychosis, co-morbid major mental illness, significant physical illness (heart disease, high blood pressure, seizures) habitual substance misuse (including alcohol)
  • ASD caused by a known genetic syndrome e.g. Fragile X or 22q11 deletion syndrome,
  • past/present treatment for epilepsy
  • Change of medication dose/start of a new pharmacological therapy in the month prior to participation

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Placebo, Arbaclofen_30, Arbaclofen_15Arbaclofen_15Dose order: Placebo, Arbaclofen 30mg, Arbaclofen 15 mg
Placebo, Arbaclofen_30, Arbaclofen_15PlaceboDose order: Placebo, Arbaclofen 30mg, Arbaclofen 15 mg
Arbaclofen_30, Placebo, Arbaclofen_15Arbaclofen_15Dose order: Arbaclofen 30mg, Placebo, Arbaclofen 15mg
Arbaclofen_30, Placebo, Arbaclofen_15Arbaclofen_30Dose order: Arbaclofen 30mg, Placebo, Arbaclofen 15mg
Arbaclofen_15, Arbaclofen_30, PlaceboPlaceboDose order: Arbaclofen 15mg, Arbaclofen 30mg, Placebo
Placebo, Arbaclofen_15, Arbaclofen_30Arbaclofen_30Dose order: Placebo, Arbaclofen 15mg, Arbaclofen 30mg
Arbaclofen_15, Arbaclofen_30, PlaceboArbaclofen_15Dose order: Arbaclofen 15mg, Arbaclofen 30mg, Placebo
Arbaclofen_30, Arbaclofen_15, PlaceboArbaclofen_30Dose order: Arbaclofen 30mg, Arbaclofen 15mg, Placebo
Arbaclofen_30, Arbaclofen_15, PlaceboPlaceboDose order: Arbaclofen 30mg, Arbaclofen 15mg, Placebo
Placebo, Arbaclofen_15, Arbaclofen_30PlaceboDose order: Placebo, Arbaclofen 15mg, Arbaclofen 30mg
Placebo, Arbaclofen_15, Arbaclofen_30Arbaclofen_15Dose order: Placebo, Arbaclofen 15mg, Arbaclofen 30mg
Placebo, Arbaclofen_30, Arbaclofen_15Arbaclofen_30Dose order: Placebo, Arbaclofen 30mg, Arbaclofen 15 mg
Arbaclofen_30, Arbaclofen_15, PlaceboArbaclofen_15Dose order: Arbaclofen 30mg, Arbaclofen 15mg, Placebo
Arbaclofen_30, Placebo, Arbaclofen_15PlaceboDose order: Arbaclofen 30mg, Placebo, Arbaclofen 15mg
Arbaclofen_15, Placebo, Arbaclofen_30Arbaclofen_15Dose order: Arbaclofen 15mg, Placebo, Arbaclofen 30mg
Arbaclofen_15, Placebo, Arbaclofen_30PlaceboDose order: Arbaclofen 15mg, Placebo, Arbaclofen 30mg
Arbaclofen_15, Arbaclofen_30, PlaceboArbaclofen_30Dose order: Arbaclofen 15mg, Arbaclofen 30mg, Placebo
Arbaclofen_15, Placebo, Arbaclofen_30Arbaclofen_30Dose order: Arbaclofen 15mg, Placebo, Arbaclofen 30mg
Primary Outcome Measures
NameTimeMethod
Neurochemical response to GABAergic stimulation.Through study completion, an average of 2 years.

Comparing brain excitation-inhibition measures (i.e., glutamate and GABA) when the GABAergic system is activated by a single oral dose of the GABA-B drug Arbaclofen versus the placebo condition.

Secondary Outcome Measures
NameTimeMethod
Brain oscillations under sensory stimulationThrough study completion, an average of 2 years.

Brain oscillations and event-related potentials will be recorded during sensory stimulation using high density electroencephalography.

Functional connectivity measures using resting state functional magnetic resonance imaging.Through study completion, an average of 2 years.

Maps of functional connectivity will be obtained for each condition and compared between adults with and without ASD.

Trial Locations

Locations (1)

King's College London

🇬🇧

London, United Kingdom

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