Autonomic Adaptation to Hydration and Brain Functioning
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Hydration
- Sponsor
- Swansea University
- Enrollment
- 12
- Primary Endpoint
- Heart-rate variability
- Status
- Completed
- Last Updated
- 7 years ago
Overview
Brief Summary
The influence of relatively small decreases in hydration status have been little studied. On two occasions subjects will be allowed to lose about 0,5% of their body weight and one occasion 300ml of water will be consumed. Functional MRI will be used to monitor brain functioning when performing a mental arithmetic task. Heart rate variability will be monitored and related to differences in brain functioning when water has and has not been drunk
Detailed Description
On two mornings twelve male participants will be exposed to a temperature of 30oC for four hours and either drink or not drink two 150ml glasses of water during that time. The subjects the participate in an fMRI protocol during which they complete a modified version of the Paced Auditory Serial Addition Test (PASAT): a challenging arithmetic task designed to elicit autonomic arousal. Throughout the morning subjects will their heart rate variability (HRV) monitored. At periodic intervals subjects will rate their thirst and mood by visual analogue scales and at the end of the PASAT task they rate its difficulty. Changes in body temperature and fluid loss (perspiration and urine) and urine osmolality will be monitored.
Investigators
David Benton
Professor
Swansea University
Eligibility Criteria
Inclusion Criteria
- •Self declared as healthy and not taking any medication
Exclusion Criteria
- Not provided
Outcomes
Primary Outcomes
Heart-rate variability
Time Frame: Differences on two testing occasions (0900 and 1300 hours) will be related to BOLD
Differences in inter-beat intervals derived from RS800 Polar heart rate monitor with a sampling rate of 1000Hz. Profile to be analysed using the Kubios software
BOLD signal
Time Frame: One hour in scanner (1300-1400 hours)
Participants will be presented with an arithmetic task, modified for use in the scanner, similar to the Paced Auditory Serial Addition Task: a 'stressful' task that measures calculation ability and is known to elicit an autonomic response 35. Pairs of two digit numbers appeared on a screen in red and participants will be required to mentally add or subtract the numbers. After 2 seconds the screens will be removed and a second screen appeared containing a correct and an incorrect answer. Participants will be required to press either a left or right button to indicate which answer was correct ). The speed of presentation is designed to be just at the level that it is possible to perform the task although it required mental effort and was 'stressful'.This allows investigation of areas of the brain associated with working memory but also those associated with emotional arousal.
Secondary Outcomes
- Ratings of ,mood and perceived effort(Rated baseline (0830 hours), 30 mins later, 280 minutes later)
- Change in body weight(Measured at baseline (0830 hours), after 290 minutes (before urinating) and 300 mintues (after urinating))
- Osmolality(Baseline (0830 hours) and after 300 minutes)