Amino acid profile and post-ingestive consequences of duckweed protein (Lemnae minor) after a single intake with pea protein as reference

Completed

Conditions: Verteringskinetiek van eiwit bij gezonde mensen
NVT

Registration Number: NL-OMON44240

Lead Sponsor: Stichting Wageningen Research -- Wageningen Food & Biobased Research

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 15

Inclusion Criteria

* Healthy as assessed by a health and lifestyle questionnaire and normal blood clinical laboratory tests for haemoglobin, kidney and liver functioning
* Age between 18 and 50 y (boundaries included) at day 01 of the study
* Body mass index (BMI) between 19 and 25 kg/m2 (boundaries included)
* Appropriate veins for blood sampling (judged by a study nurse or medical doctor)
* Willing to abstain from blood donation one month before and during the study
* Willing to abstain from the use of protein supplements during the study period
* Signed informed consent
* Willing to comply with the study procedures

Exclusion Criteria

* Any metabolic, gastrointestinal, inflammatory or chronic disease (such as diabetes, anaemia, hepatitis, cardiovascular disease)
* History of gastro-intestinal surgery
* History of liver dysfunction (cirrhosis, hepatitis) or liver surgery
* Kidney dysfynction (eGFR <60 ml/min)
* Any concomitant medication that may influence the study results, such as gastric acid inhibitors or laxatives (occasional use of paracetamol is allowed)
* Intensive sporting activities (>16 hours per week)
* Current smoker
* Alcohol intake *3 glasses of alcoholic beverages per day
* Pregnant or lactating or wishing to become pregnant in the period of the study (self-reported)
* Use of hard drugs
* Known allergies towards the products used in the study
* Not willing to consume chicken bouillon
* Participation in another clinical trial at the same time
* Being an employee of the department of Human Nutrition of Wageningen University or the Consumer Science & Health group of Wageningen Food & Biobased Research

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The main study parameter is the peak value and total amount of amino acids<br /><br>measured in the serum before (t=0 min) and at the various time points after<br /><br>consumption of the two proteins (until t=180 min). </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Secondary outcomes are the glucose and insulin levels in the blood samples in<br /><br>the same time period of three hours (t=0; t=15, t=30 * t=180) after consumption<br /><br>of the two protein products. Aural temperature, heart rate and blood pressure<br /><br>will be measured before (t=0 min) and after consumption (t=180 min). Finally,<br /><br>subjects will report gastro-intestinal complaints on the test day (consumption<br /><br>of the protein soup) and the subsequent three days via a diary. </p><br>