Quantify the mesenchymal population and the osteogenic differentiation of bone marrow cells
Withdrawn
- Conditions
- orthopedic differentiation of stem cells10023213
- Registration Number
- NL-OMON41207
- Lead Sponsor
- Academisch Medisch Centrum
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Withdrawn
- Sex
- Not specified
- Target Recruitment
- 10
Inclusion Criteria
Patients
- in need of an autologous bone graft harvested of the (anterior or posterior) iliac crest for orthopaedic reason;
- understand study proposal and sign informed consent;
- > 18 years old
Exclusion Criteria
Subjects:
- participating in other studies
- suffering from an autoimmune disease
- suffering from an infection
- suffering from a malignancy
- receiving or received chemotherapy / other bone marrow suppression medicine < 1 year ago
- suffering or suffered from a hematopoietic disease < 1 year ago
- an other known malignancy
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Avarage number of stem cells available in a bone marrow aspirate and the<br /><br>different cell typesin %.</p><br>
- Secondary Outcome Measures
Name Time Method <p>Number of stem cells with bone or vascular generating potentials (through:<br /><br>CD34, CD45, CD90, CD105, CD146, CD271 markers)<br /><br>Colony forming potentials of stem cells (through CFU-F analysis)<br /><br>Genexpressie of the osteoprogenitors (through PCR analyse)<br /><br>Other baseline parameters will be noted to detect potential confounders: sex,<br /><br>smoking, current diets, age and body weight.<br /><br><br /><br>Study parameters will be compared between patients and also to the existing<br /><br>literature.</p><br>