MedPath

Trilaciclib, a CDK 4/6 Inhibitor, in Patients Receiving FOLFOXIRI/Bevacizumab for Metastatic Colorectal Cancer (mCRC):

Phase 3
Terminated
Conditions
Colorectal Cancer Metastatic
Myelosuppression-Adult
Chemotherapeutic Toxicity
Interventions
Drug: Placebo
Registration Number
NCT04607668
Lead Sponsor
G1 Therapeutics, Inc.
Brief Summary

This was a randomized, double-blind, placebo-controlled, global, multicenter, Phase 3 trial evaluating the impact of trilaciclib on myelopreservation and anti-tumor efficacy when administered prior to FOLFOXIRI/bevacizumab in patients with pMMR/MSS mCRC who have not received systemic therapy for metastatic disease.

Detailed Description

Patients were randomly assigned (1:1) to receive placebo or trilaciclib on Days 1 and 2 administered intravenously (IV) prior to FOLFOXIRI/bevacizumab in 14-day cycles for up to 12 cycles (Induction).

Following completion of Induction, patients continued in Maintenance, where they received trilaciclib or placebo per randomization allocation at study entry. Trilaciclib/placebo will be administered prior to infusional-5FU/leucovorin/bevacizumab at the same dose and schedule used during Induction. The patient continued to receive treatment on study until disease progression, unacceptable toxicity, withdrawal of consent, discontinuation by Investigator, or the end of the trial, whichever occurs first. Treatment cycles occurred consecutively without interruption, except when necessary to manage toxicities or for administrative reasons.

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
326
Inclusion Criteria
  1. Age ≥ 18 years of age at the time of signing the informed consent. Patients > 70 years of age must have a G8 Health State Screening Tool (geriatric screening tool) score > 14.
  2. Proficient mismatch repair/microsatellite stable (pMMR/MSS), histologically or cytologically-confirmed adenocarcinoma of the colon or rectum. Patients with any BRAF or KRAS mutation status (wild type or mutant) are eligible. If historical pMMR/MSS and/or BRAF V600E mutational status are not known, a tumor specimen (archival or fresh biopsy) must be sent for testing and results must be available at the time of randomization in interactive web response system (IWRS). If testing cannot be completed using a standard clinical assay performed institutionally/locally, the tumor specimen may be sent to the Sponsor's designated central laboratory for analysis; only historical KRAS mutational status will be collected (ie, no testing required prior to study entry). Note: Any sample sent for MSS/BRAF analysis will be in addition to that required per Inclusion Criterion 5.
  3. Unresectable and measurable or metastatic colorectal cancer per RECIST v1.1
  4. ECOG performance status of 0 to 1
  5. A formalin-fixed paraffin-embedded (FFPE) tumor specimen (from archival or fresh biopsy) with an associated pathology report documenting pMMR/MSS mCRC must be confirmed to be available to send to the Sponsor for planned retrospective biomarker analyses (tissue requirements are provided in the associated laboratory manual).
  6. Hemoglobin ≥ 9.0 g/dL in the absence of RBC transfusion or ESA administration within 14 days prior to first dose of trilaciclib/placebo
  7. Absolute neutrophil count (ANC) ≥ 1.5 × 10^9 /L
  8. Platelet count ≥ 100 × 10^9 /L
  9. Estimated glomerular filtration rate (eGFR) ≥ 30 mL/minute/1.73m^2
  10. Total bilirubin ≤ 1.5 × upper limit of normal (ULN)
  11. AST, ALT, and alkaline phosphatase ≤ 3 × ULN for patients without liver or bone metastases; AST, ALT and alkaline phosphatase ≤ 5 × ULN in the presence of liver metastases; AST and ALT ≤ 3 x ULN and alkaline phosphatase ≤ 5 × ULN in the presence of bone metastases
  12. Resolution of nonhematologic toxicities from prior therapy or surgical procedures to ≤ Grade 1 or baseline (except alopecia)
  13. Urine dipstick protein < 2+. If ≥ 2+ at Screening, then a 24-hour urine collection must be done to demonstrate ≤ 1 g of protein/24 hours
  14. Contraceptive use by men or women should be consistent with local regulations regarding the methods of contraception for those participating in clinical studies. Please see Section 17.4 for detailed instructions on methods of contraception requirements.
  15. Capable of giving signed informed consent which includes compliance with the requirements and restrictions listed in the informed consent form (ICF) and in this protocol.
Exclusion Criteria
  1. Prior systemic therapy for mCRC. Patients who received adjuvant/neoadjuvant therapy (ie, treatment with curative intent) for colorectal cancer are eligible if it has been ≥ 6 months between the last dose of systemic chemotherapy and the date of informed consent.
  2. Any radiotherapy, chemotherapy, immunotherapy, biologic, investigational, or hormonal therapy for cancer treatment (except for adjuvant hormonal therapy for breast cancer or prostate cancer defined as M0 disease or PSA persistence/recurrence without metastatic disease) within 3 weeks prior to the first dose of trilaciclib/placebo.
  3. Receipt of any low-dose systemic chemotherapeutic agent (e.g., low-dose methotrexate for rheumatoid arthritis) administered for a nononcologic purpose within 3 weeks prior to the first dose of trilaciclib/placebo.
  4. Presence of central nervous system (CNS) metastases/leptomeningeal disease requiring immediate treatment with radiation therapy or steroids (i.e., patient must be off steroids administered for brain metastases for at least 14 days prior to the first dose of trilaciclib/placebo).
  5. QTcF interval > 450 msec (males) or > 470 msec (females) at screening. For patients with ventricular pacemakers, QTcF > 500 msec.
  6. Personal or family history of long QT syndrome
  7. Symptomatic peripheral neuropathy
  8. History of interstitial lung disease (ILD)
  9. Uncontrolled hypertension (blood pressure ≥ 150/90mm Hg)
  10. Clinically significant (i.e., active) cardiovascular disease at the time of signing the informed consent; for example cerebrovascular accidents (≤ 6 months before the first dose of trilaciclib/placebo), myocardial infarction (≤ 6 months before the first dose of trilaciclib/placebo), unstable angina, serious cardiac arrhythmia requiring medication, or uncontrolled symptomatic congestive heart failure [Class II or higher as defined by the New York Heart Association [NYHA] functional classification system])
  11. Serious, non-healing wound, ulcer, or bone fracture
  12. Major surgical procedure, open biopsy, or significant traumatic injury within 28 days prior to study treatment start, or anticipation of the need for major surgical procedure during the course of the study.
  13. Known serious active infection (e.g., human immunodeficiency virus [HIV], hepatitis B or C, tuberculosis, etc.)
  14. Known Gilbert's Syndrome or homozygous for the UGT1A1*28 allele. UGT1A1 genotyping is not required for this study.
  15. Chronic inflammatory bowel disease and/or active intestinal obstruction. Patients should not be treated until the intestinal obstruction has resolved.
  16. Previous history of significant/severe hemorrhage, within 1 month before randomization. History of previous abdominal fistula or gastrointestinal perforation within 6 months before randomization
  17. Known history of bleeding diathesis or coagulopathy
  18. INR > 1.5 within 14 days prior to starting study treatment. EXEMPTION: patients on full anticoagulation must have an in-range INR (usually between 2 to 3) if INR is used for monitoring. Any anticoagulation therapy must be at stable dosing prior to enrollment.
  19. Ongoing or anticipated treatment with potent cytochrome inhibitors CYP450 3A4 (such as ketoconazole) or inducers (such as rifampicin, carbamazepine, phenobarbital, phenytoin or St. John's wort). Irinotecan should not be delivered concurrently.
  20. Patients with ongoing or anticipated treatment with sorivudine or its chemically related analogues, such as brivudine.
  21. Chronic, daily treatment with high-dose aspirin (> 325 mg/day)
  22. Prior allogeneic or autologous hematopoietic stem cell or bone marrow transplantation
  23. Receipt of any live attenuated vaccines within 4 weeks prior to first dose of study treatment
  24. Known hypersensitivity to any of the drugs used in this study
  25. Pregnant or lactating women
  26. Legal incapacity or limited legal capacity
  27. Other uncontrolled serious chronic disease or psychiatric condition that in the Investigator's opinion could affect patient safety, compliance, or follow-up in the protocol
  28. Any contraindications to the administration of FOLFOXIRI and bevacizumab at the discretion of the investigator.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
trilaciclib + FOLFOXIRI/bevacizumabTrilaciclibDuring Induction the following study drugs are administered on Day 1: Irinotecan - IV, Oxaliplatin - IV, Leucovorin- IV, Fluorouracil - continuous infusion (CI) over 46 to 48 hours beginning on Day 1, Bevacizumab - IV Following completion of Induction, patients will continue in Maintenance, where they will continue to receive trilaciclib per randomization allocation at study entry. Trilaciclib will be administered prior to infusional- 5FU/leucovorin/bevacizumab at the same dose and schedule used during Induction.
placebo + FOLFOXIRI/bevacizumabPlaceboThe subjects in the placebo arm will follow the same schedule as the trilaciclib arm, but will receive placebo instead of trilaciclib.
Primary Outcome Measures
NameTimeMethod
Duration of Severe Neutropenia (DSN)Cycles 1 to 4 (14-day cycles up to 56 days)

The DSN was defined as the number of days for the first severe neutropenia (SN) event in Cycles 1, 2, 3, or 4 for participants who had at least one SN event in the first 4 cycles of Induction. It was calculated as the days from the date of the first absolute neutrophil count (ANC) value of \< 0.5 × 10\^9/L to the date of the first ANC value ≥ 0.5 × 10\^9/L where no additional ANC values \< 0.5 × 10\^9/L were observed for the remainder of that cycle.

Occurrence of Severe Neutropenia (SN) During InductionInduction Period, cycles 1-12 (14-day cycles up to 168 days)

Severe neutropenia was defined as the absolute neutrophil count (ANC) laboratory value that met the Common Terminology Criteria for Adverse vents (CTCAE) criteria for ≥ Grade 4 toxicity (ie, ANC \< 0.5 × 10\^9/L in SI Unit)

Secondary Outcome Measures
NameTimeMethod
Overall Survival (OS)Up to 52 months

Overall survival is defined as the time from the date of the first dose of study treatment to the date of death from any cause.

Additional Myelopreservation MeasuresThrough Induction Period - on average 24 weeks (up to 12 cycles) of FOLFOXIRI/bevacizumab

To assess the effects of trilaciclib on additional measures of the neutrophil lineage compared with placebo in patients receiving FOLFOXIRI/bevacizumab for pMMR/MSS mCRC as measured by the number of SN events, granulocyte-colony stimulating factor (G-CSF) administration and febrile neutropenia (FN) adverse events (AE)

Red Blood Cell LineageThrough Induction Period - on average 24 weeks (up to 12 cycles) of FOLFOXIRI/bevacizumab

To assess the effects of trilaciclib on the red blood cell (RBC) lineage compared with placebo in patients receiving FOLFOXIRI/bevacizumab for pMMR/MSS mCRC by measure of Grade 3 or 4 decreased hemoglobin laboratory values, RBC transfusions on or after Week 5, and erythropoiesis-stimulating agents (ESA) administration

Platelet LineageThrough Induction Period - on average 24 weeks (up to 12 cycles) of FOLFOXIRI/bevacizumab

To assess the effects of trilaciclib on the platelet lineage compared with placebo in patients receiving FOLFOXIRI/bevacizumab for pMMR/MSS mCR by measure of Grade 3 or 4 decreased platelet count laboratory values and Platelet transfusions.

Multiple LineageThrough Induction Period - on average 24 weeks (up to 12 cycles) of FOLFOXIRI/bevacizumab

To assess the effects of trilaciclib on multiple lineages compared with placebo in patients receiving FOLFOXIRI/bevacizumab for pMMR/MSS mCRC by measure of Grade 3 or 4 hematologic lab values.

Standard of Care DosingThrough Induction Period - on average 24 weeks (up to 12 cycles) of FOLFOXIRI/bevacizumab

To assess the effects of trilaciclib on standard of care dosing compared with placebo in patients receiving FOLFOXIRI/ bevacizumab for pMMR/MSS mCRC by measure of all-cause dose reductions or cycle delays and relative dose intensity for FOLFOXIRI/bevacizumab

Healthcare UtilizationThrough Induction Period - on average 24 weeks (up to 12 cycles) of FOLFOXIRI/bevacizumab

To assess the effects of trilaciclib on healthcare utilization compared with placebo in patients receiving FOLFOXIRI/ bevacizumab for pMMR/MSS mCRC by measure of hospitalizations and antibiotic use.

Best Overall Response (BOR)Through Induction Period - on average 24 weeks (up to 12 cycles) of FOLFOXIRI/bevacizumab

Best overall response (BOR) will be determined using all visit responses prior to or on the date of (i) radiographic disease progression; (ii) withdrawal of consent to obtain scans; (iii) death; (iv) lost to follow-up; or (v) initiation of subsequent anti-cancer therapy other than the study drugs, whichever is earlier will be based on RECIST v1.1.

Objective Response Rate (ORR)Assessed from the day of the first dose of the study drug, through 30 days after the last dose of the study drug, up to 115 weeks

ORR was defined as the percentage of participants in the analysis population who had a Complete Response (CR) defined as a disappearance of all target lesions with pathological lymph nodes having a reduction in short axis to \<10 mm or Partial Response (PR) defined as at least a 30% decrease in the sum of diameters of target lesions, using the baseline sum diameters as a reference based on RECIST v1.1.

Duration of Objective Response (DOR)Assessed from the day of the first dose of the study drug, through 30 days after the last dose of the study drug, up to 115 weeks

DOR is the time between first objective response of CR or PR and the first date that progressive disease is objectively documented or death, whichever comes first.

Progression Free Survival (PFS)Assessed from the day of the first dose of the study drug, through 30 days after the last dose of the study drug, up to 115 weeks

PFS is defined as the time from the date of randomization until the date of documented radiologic disease progression per RECIST v1.1 or death due to any cause, whichever comes first.

Quality of Life/ Effects on Chemotherapy-Induced FatigueThrough Induction Period- on average 24 weeks (up to 12 cycles) of FOLFOXIRI/bevacizumab

To assess the effects of trilaciclib on chemotherapy-induced fatigue compared with placebo in patients receiving FOLFOXIRI/bevacizumab for pMMR/MSS mCRC, as measured by Time To First Confirmed Deterioration of Fatigue (TTCD-fatigue) during Induction, as measured by the FACIT-F (Functional Assessment of Chronic Illness Therapy-Fatigue).

Number of Participants With Reported Adverse Events to Measure Safety and TolerabilitySafety was assessed from the day of the first dose of the study drug, through 30 days after the last dose of the study drug, up to 115 weeks

To assess the safety and tolerability of trilaciclib compared with placebo in patients receiving FOLFOXIRI/ bevacizumab for pMMR/MSS mCRC by measure of occurrence and severity of AEs by National Cancer Institute-Common Terminology Criteria for Adverse Events (NCI-CTCAE) v5.0, changes in laboratory parameters, vital signs and electrocardiogram (ECG) parameters, grade 3 or 4 abnormalities in chemistry laboratory parameters, and study treatment discontinuation due to AEs.

Trial Locations

Locations (82)

AZ Oncology Associates - HOPE

🇺🇸

Tucson, Arizona, United States

Keck Medical Center of USC Pasadena

🇺🇸

Los Angeles, California, United States

Florida Cancer Specialists - Panhandle

🇺🇸

Tallahassee, Florida, United States

Northside Hospital - Georgia Cancer Specialists

🇺🇸

Atlanta, Georgia, United States

Beverly Hills Cancer Center

🇺🇸

Beverly Hills, California, United States

University of Massachusetts Memorial Medical Center

🇺🇸

Worcester, Massachusetts, United States

UT Southwestern Medical Center

🇺🇸

Dallas, Texas, United States

Bekes Megyei Kozponti Korhaz Pandy Kalman Tagkorhaza

🇭🇺

Gyula, Hungary

Hospital Universitario Miguel Servet

🇪🇸

Zaragoza, Spain

Treatment-Diagnostic Center of Private Enterprise of PPC Atsynus

🇺🇦

Kropyvnytskyi, Ukraine

ASL Regionale Piemonte - Ospedale Santo Spirito Casale Monferrato (Ospedale di Casale Monferrato)

🇮🇹

Casale Monferrato, Alessandria, Italy

Hospital Universitario 12 de Octubre

🇪🇸

Madrid, Spain

Florida Cancer Specialists (South Region)

🇺🇸

Fort Myers, Florida, United States

The Oncology Institute of Hope & Innovation\ Innovative Clinical Research Institute

🇺🇸

Whittier, California, United States

Florida Cancer Specialists NORTH

🇺🇸

Fort Myers, Florida, United States

Illinois Cancer Specialists

🇺🇸

Arlington Heights, Illinois, United States

Mid-Florida Hematology & Oncology Centers, P.A.

🇺🇸

Orange City, Florida, United States

Florida Cancer Specialists

🇺🇸

Saint Petersburg, Florida, United States

Gettysburg Cancer Center

🇺🇸

Gettysburg, Pennsylvania, United States

The First Affiliated Hospital of Zhejiang University

🇨🇳

Hangzhou, Zhejiang, China

Millennium Oncology

🇺🇸

Kingswood, Texas, United States

Inova Schar Cancer Institute

🇺🇸

Fairfax, Virginia, United States

Jilin Provincial Tumor Hospital

🇨🇳

Changchun, Jilin, China

Henan Cancer Hospital

🇨🇳

Zijingshan, Henan, China

Onc and Hem Assoc of SW VA

🇺🇸

Roanoke, Virginia, United States

Wuhan Union Hospital

🇨🇳

Wuhan, Hubei, China

Jinan Central hospital

🇨🇳

Shandong, China

The Affiliated Tumor Hospital of Harbin Medical University

🇨🇳

Heilongjiang, China

Orszagos Onkologiai Intezet

🇭🇺

Budapest, Hungary

Xuzhou Central hospital

🇨🇳

Xuzhou, China

Zhejiang Cancer Hospital

🇨🇳

Hangzhou, Zhejiang, China

Zhongshan Hospital Fudan University

🇨🇳

Shanghai, China

First Affiliated Hospital of Zhengzhou University

🇨🇳

Zhengzhou, China

Bacs-Kiskun Megyei Oktatokorhaz

🇭🇺

Kecskemet, Hungary

SzSzB Megyei Korhazak es Egyetemi Oktatokorhaz

🇭🇺

Nyiregyhaza, Hungary

Azienda Ospedaliera Universitaria Policlinico Tor Vergata

🇮🇹

Rome, Roma, Italy

Fondazione IRCCS CA' Granda Ospedale Maggiore Policlinico

🇮🇹

Cremona, Italy

Azienda Ospedaliera Universitaria Careggi

🇮🇹

Firenze, Italy

Fondazione Policlinico Universitario Agostino Gemelli IRCCS

🇮🇹

Rome, Roma, Italy

ICO l'Hospitalet - Hospital Duran i Reynals

🇪🇸

Barcelona, Spain

Szpitale Pomorskie spółka z ograniczoną odpowiedzialnością

🇵🇱

Gdynia, Poland

Hospital Universitari Vall d'Hebron

🇪🇸

Barcelona, Spain

Szpital Specjalistyczny im. L.Rydygiera w Krakowie

🇵🇱

Krakow, Poland

Centrum Medyczne Pratia Poznan

🇵🇱

Skórzewo, Poland

Hospital Clinic de Barcelona

🇪🇸

Barcelona, Spain

Hospital Universitario Virgen del Rocio

🇪🇸

Sevilla, Spain

Hospital Universitario Lucus Augsti

🇪🇸

Lugo, Spain

Hospital Universitari Arnau de Vilanova

🇪🇸

Lleida, Spain

Hospital General Universitario Gregorio Marañon

🇪🇸

Madrid, Spain

Hospital Universitario La Paz

🇪🇸

Madrid, Spain

Hospital Universitario HM Madrid Sanchinarro

🇪🇸

Madrid, Spain

CI Cherkasy Regional Oncological Dispensary of CRC

🇺🇦

Cherkasy, Ukraine

Dnipropetrovsk City Multispecialty Clinical Hospital #4

🇺🇦

Dnipro, Ukraine

Hospital Universitario Puerta de Hierro Majadahonda

🇪🇸

Madrid, Spain

Hospital Universitario Virgen Macarena

🇪🇸

Sevilla, Spain

Hospital Clinico Universitario de Valencia

🇪🇸

Valencia, Spain

MI Regional Clinical Oncologycal Dispensary

🇺🇦

Dnipro, Ukraine

Limited Liability Company "Medical Center named by Academician Yuriy Prokopovich Spizhenko"

🇺🇦

Kapitanivka, Ukraine

Communal Non-profit Enterprise Regional Center of Oncology, Kharkiv NMU

🇺🇦

Kharkiv, Ukraine

CNE Prof. O.O. Shalimov Kharkiv City Clinical Hospital #2 of KCC

🇺🇦

Kharkiv, Ukraine

CI Kryvyi Rih Oncological Dispensary of DRC

🇺🇦

Kryvyi Rih, Ukraine

Communal Institution Odesa Regional Clinical Hospital; Department of Surgery

🇺🇦

Odesa, Ukraine

Medical Center Asklepion LLC

🇺🇦

Kyiv, Ukraine

Communal Enterprise Volyn Regional Medical Center of Oncology of Volyn Regional Council

🇺🇦

Lutsk, Ukraine

Medical Center of Limited Liability Company Medical Center Concilium Medical

🇺🇦

Kyiv, Ukraine

University Hospital of Sumy State University

🇺🇦

Sumy, Ukraine

The Christie

🇬🇧

Manchester, Greater Manchester, United Kingdom

Barts Hospital

🇬🇧

London, Greater London, United Kingdom

CNE CCCH of Uzh CC Oncological Center, Ther Dept, SHEI UNU

🇺🇦

Uzhgorod, Ukraine

Royal Free Hospital

🇬🇧

London, Greater London, United Kingdom

Velindre Cancer Centre

🇬🇧

Cardiff, South Glamorgan, United Kingdom

Georgetown University - Lombardi Comprehensive Cancer Center

🇺🇸

Washington, District of Columbia, United States

Hospital Universitario Ramon y Cajal

🇪🇸

Madrid, Spain

Mrukmed Lekarz Beata Madej Mruk i Partner Spółka Partnerska Oddział nr 1 w Rzeszowie

🇵🇱

Rzeszów, Poland

Centrum Zdrowia MDM

🇵🇱

Warszawa, Poland

Medical center "Oncolife" LLC

🇺🇦

Zaporizhzhia, Ukraine

Boston Medical Center

🇺🇸

Boston, Massachusetts, United States

Mayo Clinic - Rochester

🇺🇸

Rochester, Minnesota, United States

Comp. Cancer Centers of Nevada

🇺🇸

Las Vegas, Nevada, United States

University of Oklahoma Health Sciences Center

🇺🇸

Oklahoma City, Oklahoma, United States

Oregon Health & Science University

🇺🇸

Portland, Oregon, United States

Tennessee Oncology

🇺🇸

Nashville, Tennessee, United States

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