leprosy ulcers and bacterial infections
Not Applicable
- Conditions
- Health Condition 1: L088- Other specified local infections of the skin and subcutaneous tissue
- Registration Number
- CTRI/2022/01/039186
- Lead Sponsor
- IADVL research grant
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- ot Yet Recruiting
- Sex
- Not specified
- Target Recruitment
- 0
Inclusion Criteria
a) Leprosy patients with plantar ulcers who are not on topical or systemic antibiotics since atleast 2 weeks
b) Age above 18 years
Exclusion Criteria
a. patients with a history of use of topical or systemic antibiotics within 2 weeks.
b. Patients with ulcer who are admitted in hospital since atleast 5 days which can alter bacterial colonization
Study & Design
- Study Type
- Observational
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Quantitative <br/ ><br>measurement of biofilm production by the Microtitre plate method (MTP) will be performed for in clinical isolates of bacteria. An optical <br/ ><br>density (OD) greater than 0.240 indicated a strong biofilm producer, an OD <br/ ><br>between 0.120â??0.240 a moderate biofilm producer and an OD of less than 0.120, <br/ ><br>a non-biofilm producer. S. aureus ATCC 25923 will be used as the control <br/ ><br>organism for biofilm production <br/ ><br>Timepoint: 3 days
- Secondary Outcome Measures
Name Time Method The antimicrobial susceptibility testing (AST) determined by the Kirby-Bauer <br/ ><br>disc diffusion method after it is incubated for 18â??24 h at 37 °C aerobically, the <br/ ><br>isolates will be classified as susceptible, intermediate and resistant as per standard <br/ ><br>guidelines.Timepoint: 24hrs