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Pharmacokinetic Drug Interactions of AEGR-733 on Lipid-lowering Agents

Phase 2
Completed
Conditions
Healthy
Registration Number
NCT00359281
Lead Sponsor
Aegerion Pharmaceuticals, Inc.
Brief Summary

This phase II, open-label research study was conducted in 129 healthy volunteers. Each subject will be given one initial oral dose of one of 7 FDA-approved medications (probe drugs), followed by a 7 day period where subjects receive the study medication AEGR-733 at 10 or 60 mg. On study day 8 subjects will receive the second oral dose of the same probe drug that was given on day 1 and a last dose of AEGR-733 (total of 7 doses).Subjects will return in 1 week for a final safety visit. Each FDA- approved probe drug will be given to ten (10) or fifteen (15) subjects.

Safety, pharmacokinetic and pharmacodynamic assessments will be performed.

Detailed Description

Objectives:

Primary: To evaluate the effects of low and high doses of AEGR-733 on the pharmacokinetics of 6 FDA-approved medications that are likely to be used in combination with AEGR-733 as assessed by:

• Pharmacokinetic parameters: Cmax, Tmax, T1/2, and AUC (area under the curve).

Secondary: To evaluate the safety of AEGR-733 in combination with other lipid lowering agents in healthy subject as assessed by:

* Changes in associated liver enzymes AST, ALT and, Alkaline Phosphatase, \& Total Bilirubin.

* Changes in all reported adverse events.

* To evaluate the effects of AEGR-733 in combination with other lipid lowering agents on the following lipids and lipoproteins: TC, LDL-C, VLDL, TG, HDL-C, ApoB and ApoAI.

4.0 STUDY DESIGN AND RATIONALE

4.1 STUDY DESIGN This is a single-center, phase II, clinical trial consisting of a eight (8) day open-label phase to assess the pharmacokinetic drug interactions of AEGR-733 on 6 probe drugs in healthy volunteers, followed by a one week safety visit. 105 subjects will be enrolled into this fixed-sequenced research study. Eligible subjects based on the screening visit will come to the GCRC for an inpatient visit (25-36 hr depending on if they come in evening before study day 1 or morning of). On the morning of study day 1, subjects will be assigned to one of 6 probe drugs(A-H below) and will take one dose of this medication. Timed blood samples will be drawn just before the administration of the probe drug and during the following times after drug administration (1,2,3,4,5,6,8,10,12,18, and 24 hrs). Prior to discharge after the 24 h blood sample, subjects will take an oral dose of AEGR-733 at 10 mg or 60 mg. Subjects will be given a 5 day supply of AEGR-733 at 10 mg or 60 mg to be taken once daily in the morning for the next 5 days (through day 7). On study day 8, subjects will take a final dose of AEGR-733 at 10 mg or 60 mg (total doses= 7) simultaneously with the same probe drug they took on day 1. Timed blood samples will be drawn just before the administration of the probe drug and AEGR-733 as well as 1,2,3,4,5,6,8,10,12,18, and 24 hours after study drug administration. After the 24 hour blood sample, subjects will be discharged. 15 subjects who participate in this study will receive dextromethorphan as the probe drug, which requires urine collection for 8 hours post dose. Blood for pharmacokinetic samples will not be collected on these subjects. Subjects receiving dextromethorphan may leave after the 8 hour urine collection at visits 2 and 3 (referred to as the inpatient visits). All subjects will come back 1 week later for a final visit to check safety lab parameters including liver transaminases and total bilirubin. Subjects will be instructed to abstain from drinking any alcoholic beverages once screened until study completion. Subjects who are not willing to comply with these requests will not be enrolled.

The FDA-approved lipid-lowering therapies will include:

A) Atorvastatin, 20 mg (n=15)and AEGR-733 10 mg B) Ezetimibe, 10 mg (n=10)and AEGR-733 10 mg C) Simvastatin, 20 mg (n=15)and AEGR-733 10 mg D) Rosuvastatin, 20 mg (n=10)and AEGR-733 10 mg E) Micronized fenofibrate, 145 mg (n=10)and AEGR-733 10 mg F) Atorvastatin, 20 mg (n=15) and AEGR-733 60 mg G) Rosuvastatin, 20 mg (n=15) and AEGR-733 60 mg H) Dextromethorphan, 30 mg (n=15) and AEGR-733 60 mg I) Extended Release Niacin, 1000 mg (n=20) and AEGR-733 10 mg

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
125
Inclusion Criteria

Males and non-pregnant/non-lactating female subjects between the ages of 18 and 70 who are in good overall health.

To be eligible for enrollment in this study, patients must meet all of the following criteria:

  1. Men and women between the ages of 18 and 70
  2. Women of child-bearing potential, that is, women not surgically sterilized and between menarche and 1 year post menopause, must test negative for pregnancy at the time of enrollment based on a urine pregnancy test and agree to use a reliable method of non-medication birth control (for example, a reliable barrier method of birth control [diaphragms with contraceptive jelly; cervical caps with contraceptive jelly; condoms with contraceptive foam; intrauterine devices]; partner with vasectomy; or abstinence) during the study and for one month following the last dose of study drug.
  3. Subjects must be in good overall health
  4. Subjects must be able to comprehend and willing to provide a signed IRB approved Informed Consent Form.
  5. Subjects must be willing to comply with all study-related procedures.
Exclusion Criteria
  1. Known atherosclerotic cardiovascular disease, including coronary disease, cerebrovascular disease, or peripheral vascular disease
  2. History of diabetes mellitus or fasting glucose > 126 mg/dL at the screening visit.
  3. History of a non-skin malignancy within the previous 5 years
  4. Renal insufficiency as defined by creatinine > 1.3 mg/dl
  5. Any major active rheumatologic, pulmonary, or dermatologic disease or inflammatory condition
  6. History of hypertension
  7. Known coagulopathy and /or elevated PT/PTT >1.5 x ULN
  8. Oral history of HIV positive
  9. Patients who have undergone any organ transplant
  10. Known active fibrotic or cirrhotic disease; ALT or AST > 1.5x ULN
  11. Any major surgery within the previous 3 months
  12. Individuals who currently use tobacco products or have done so in the previous 30 days
  13. History of drug abuse (< 3 years)
  14. Regular use of alcoholic beverages (> 7 drinks/day)
  15. Subjects who do not agree to abstain from consuming alcoholic beverages during the entire study duration.
  16. Body mass index (BMI) > 30 kg/m2 or < 18.5 kg/m2
  17. Participation in an investigational drug study within 6 weeks prior to the screening visit
  18. Serious or unstable medical or psychological conditions that, in the opinion of the investigator, would compromise the subject's safety or successful participation in the study will be excluded.
  19. Currently taking any prescription, including oral contraceptives, or OTC medication regularly that cannot be stopped for at least 30 days prior to enrollment until completion of the study
  20. Regular consumers of grapefruit juice, or have taken any medications known to be metabolized by CYP 3A4 within 4 weeks prior to the screening visit (ie. SSRIs, anti-fungals, anti-biotics, etc)
  21. History of myalgia with a statin or unknown hypersensitivity to any statin, zetia, AEGR-733, or fenofibrate.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Primary Outcome Measures
NameTimeMethod
AUC0-t Atorvastatin Acid (Lomitapide 60 mg)0 to 24 hours

Geometric Mean Ratio ln(AUC0-t) Day 8/Day 1 for atorvastatin acid (Lomitapide 60 mg)

AUC0-t Nicotinic Acid0 to 24 hours

Geometric Mean Ratio ln(AUC0-t) Day 8/Day 1 for nicotinic acid

AUC0-t Simvastatin0 to 24 hours

Geometric Mean Ratio ln(AUC0-t) Day 8/Day 1 for simvastatin

AUC0-t Simvastatin Acid0 to 24 hours

Geometric Mean Ratio ln(AUC0-t) Day 8/Day 1 for simvastatin acid

AUC0-t Total Ezetimibe0 to 24 hours

Geometric Mean Ratio ln(AUC0-t) Day 8/Day 1 for total ezetimibe

AUC0-t Rosuvastatin (Lomitapide 10 mg)0 to 24 hours

Geometric Mean Ratio ln(AUC0-t) Day 8/Day 1 for rosuvastatin (Lomitapide 10 mg)

AUC0-t Fenofibric Acid0 to 24 hours

Geometric Mean Ratio ln(AUC0-t) Day 8/Day 1 for fenofibric acid

Area Under Concentration-time Curve From 0 to Last Measureable Concentration (AUC0-t) Atorvastatin Acid (Lomitapide 10 mg)0 to 24 hour

Geometric Mean Ratio ln(AUC0-t) Day 8/Day 1 for atorvastatin acid (Lomitapide 10 mg)

AUC0-t Rosuvastatin (Lomitapide 60 mg)0 to 24 hours

Geometric Mean Ratio ln(AUC0-t) Day 8/Day 1 for rosuvastatin (Lomitapide 60 mg)

AUC0-t Nicotinuric Acid0 to 24 hours

Geometric Mean Ratio ln(AUC0-t) Day 8/Day 1 for nicotinuric acid

Secondary Outcome Measures
NameTimeMethod
Percent Change From Baseline in Low-density Lipoprotein Cholesterol (LDL-C)Baseline to Day 8

Percent change from Baseline in LDL-C

Trial Locations

Locations (1)

University of Pennsylvania

🇺🇸

Philadelphia, Pennsylvania, United States

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