Different Susceptibility to SARS CoV-2 Infection Among Health Care Workers Highly Exposed to COVID-19.

Completed

• Conditions: Susceptibility, Disease; Immune Response; Health Care Worker Patient Transmission; Receptor Site Alteration

• Registration Number: NCT04402827
• Lead Sponsor: Asociacion para el Estudio de las Enfermedades Infecciosas

Brief Summary

The primary objective of this study is to establish differences in susceptibility to SARS CoV-2 infection among health care workers (HCW) highly exposed to patients with COVID-19 diagnosis. To ascertain this issue, we evaluated:

* Changes in receptor polymorphism (ACE2 and CD26 receptor study.

* SARS-CoV-2 CD4/CD8 T cell response (CTL)

* Different KIR phenotypes

Detailed Description

Only 24% of health care workers (HCW) had developed inmunological response to SARS CoV-2 infection in one centre attending thousands of COVID-19 patients, and with shorteness of personal protective equipments. Our hypothesis is that this relatively low number of infected HCW could be secondary to:

1. Differences in susceptibility to infection mediated by changes in viral receptors. Thus, it is important to characterize and genotyping the main receptor for SARS-CoV-2, ACE2, and other related receptor, such as CD26.

2. Increased cellular immune response, offering cross-immunity against SARS CoV-2 infection by previous exposure to other coronavirus or respiratory pathogens. A specific CD4/CD8 T cell response to viral peptides could respond this question

3. Specific KIR phenotypes (Killer Immunoglobulin-like Receptors): Natural killer cells (NK) response to alterations of class I HLA molecules presented in infected cells. An increase in class I HLA expression could lead to an increase in NK activation by increasing its ability to produce IFN-gamma.

Study Timeline

• Study First Submitted: 2020-05-23
• Study First Submitted QC: 2020-05-23
• Study First Posted: 2020-05-27
• Study Start: 2020-08-01
• Primary Completion: 2021-08-30
• Study Completion: 2021-09-30
• Status Verified: 2021-10
• Last Update Submitted: 2021-10-23
• Last Update Posted: 2021-10-26

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 140

Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Susceptibility to SARS CoV-2 infection according to ACE2 receptor	1 month	ACE2 analysis
Susceptibility to infections according to KIR phenoytpes	2 months	Analysis of KIR in NK cells
Cellular immune response to SARS CoV-2 infection	1 month	Activation of CD4-CD8 by viral peptides

Secondary Outcome Measures

Name	Time	Method
Cellular immune response in HCW with positive IgG against SARS CoV-2	1 month	Activation of CD4-CD8 by viral peptides
Characteristics of exposure in time and intensity of HCW with SARS CoV-2 infection	1 month	Survey

Trial Locations

Locations (1)

Hospital Ramon y Cajal; 🇪🇸 Madrid, Spain