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Efficacy and Safety APT-1011 in Adult Subjects With Eosinophilic Esophagitis (EoE) (FLUTE-2)

Phase 3
Completed
Conditions
Eosinophilic Esophagitis
Interventions
Drug: Placebo oral tablet
Procedure: Esophagogastroduodenoscopy
Registration Number
NCT04281108
Lead Sponsor
Ellodi Pharmaceuticals, LP
Brief Summary

This is a 2-part randomized, double-blind, placebo-controlled study followed by an open-label extension (OLE) of APT-1011 in adults with EoE.

Part A will evaluate the efficacy and safety of APT-1011 3 mg administered hora somni (HS; at bedtime) for the induction of response to treatment (histologic and symptomatic) over 12 weeks.

Part B will evaluate histological relapse-free status in patients re-randomized to continue APT-1011 or placebo (active treatment withdrawal) until Week 52.

Part C, the OLE, will continue until regulatory approval of APT-1011 or Sponsor termination of the study.

Detailed Description

This is a 2-part randomized, double-blind, placebo-controlled study followed by an OLE of APT-1011 in adults with EoE.

Part A will evaluate the efficacy and safety of APT-1011 3 mg administered HS for the induction of response to treatment (histologic and symptomatic) over 12 weeks.

At Week 14, subjects will move into Part B. Subjects with histological response to APT-1011, defined as ≤6 peak eos/HPF, will be re-randomized to continue APT-1011 or receive placebo (active treatment withdrawal). APT-1011 histological non-responders will continue APT-1011, and placebo histological non-responders will receive APT-1011 3 mg HS. Placebo histological responders will continue placebo. The double-blind will be sustained throughout Part B. Histological responder status will be determined at the time of esophagogastroduodenoscopy (EGD) in Part B (at or prior to Week 52, depending on unscheduled EGDs performed when the Investigator deems the subject's symptoms necessitate EGD) and is defined as ≤6 peak eos/HPF.

At Week 52, subjects may enter Part C, an open-label single-arm extension phase, and continue study drug uninterrupted. Part C will terminate upon regulatory approval of APT-1011 or Sponsor termination of the study.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
143
Inclusion Criteria
  1. Male or female ≥18 years of age at the time of informed consent or assent

  2. Each subject must read, understand, and provide consent on the ICF for this study and be willing and able to adhere to study-related treatment regimens, procedures, and visit schedule

  3. Diagnosis or presumptive diagnosis of EoE that is confirmed during the Screening period by histology that demonstrates ≥15 peak eos/HPF. In order to ensure that a diagnosis can be made, at least 6 biopsies should be taken including both proximal and distal specimens (at least 3 each). Mid-esophageal biopsies are not required (optional). HPF will be defined as a standard area of 235 square microns in a microscope with 40x lens (0.3 mm^2) and 22 mm ocular.

    1. Esophagogastroduodenoscopies and biopsies are to be obtained during the Screening period
    2. Biopsies will be read by a central pathologist
    3. Esophagogastroduodenoscopies and biopsies performed outside the study will not be accepted to meet eligibility criteria
    4. Optional biopsies may be taken and processed locally for local use, if specified in the local ICF. If serious pathology is unexpectedly encountered biopsies of such lesions must be processed locally
  4. Have a subject-reported history of ≥6 episodes of dysphagia in the 14 days prior to baseline

  5. Completion of the daily diary on at least 11 out of the 14 days during the 2-week Baseline Symptom Assessment

Exclusion Criteria
  1. Have known contraindication, hypersensitivity, or intolerance to corticosteroids
  2. Have a contraindication to, or factors that substantially increase the risk of, EGD procedure or esophageal biopsy or have narrowing of the esophagus that precludes EGD with a standard 9 mm endoscope
  3. Have history of an esophageal stricture requiring dilatation within the 12 weeks prior to Screening
  4. Have any physical, mental, or social condition or history of illness or laboratory abnormality that in the Investigator's judgment might interfere with study procedures or the ability of the subject to adhere to and complete the study or increase the safety risk to the subject such as uncontrolled diabetes or hypertension
  5. History or presence of oral or esophageal mucosal infection whilst using inhaled or nasal corticosteroids
  6. Have any mouth or dental condition that prevents normal eating (excluding braces)
  7. Have any condition affecting the esophageal mucosa or altering esophageal motility other than EoE, including erosive esophagitis (grade B or higher as per the Los Angeles Classification of Gastroesophageal Reflux Disease; hiatus hernia longer than 3 cm, Barrett's esophagus, and achalasia)
  8. Use of systemic (oral or parenteral) corticosteroids within 60 days before Screening, use of swallowed corticosteroids within 30 days before Screening
  9. Initiation of either inhaled or nasal corticosteroids or high-potency dermal topical corticosteroids within 30 days before Screening
  10. Use of calcineurin inhibitors or purine analogues (azathioprine, 6-mercaptopurine) in the 12 weeks before Screening
  11. Use of potent cytochrome P450 (CYP) 3A4 inhibitors (eg, ritonavir and ketoconazole) in the 12 weeks before Screening
  12. Initiation of an elimination diet or elemental diet within 30 days before Screening (diet must remain stable after signing ICF)
  13. Morning (07:00 to 09:00, or as close to that window as possible) serum cortisol level ≤5 μg/dL (138 nmol/L) that is not responsive to adrenocorticotropic hormone (ACTH) stimulation: defined as a serum cortisol level <16 μg/dL (440 nmol/L) at 60 minutes with ACTH stimulation test using 250 μg cosyntropin (i.e., an abnormal result on the ACTH stimulation test)
  14. Use of biologic immunomodulators in the 24 weeks before Screening (allergy desensitization injection or oral therapy is allowed as long as the course of therapy is not altered during the study period)
  15. Subjects who have initiated, discontinued, or changed dosage regimen of histamine H2 receptor antagonists, antacids or antihistamines for any condition such as gastro-esophageal reflux disease within 4 weeks before qualifying endoscopy during Screening. If already receiving these drugs, the dosage must remain constant throughout the study
  16. Subjects who have changed dosage regimen of PPIs within 8 weeks before qualifying endoscopy. If already receiving PPIs, the dosage must remain constant throughout the study
  17. Infection with hepatitis B, hepatitis C, or human immunodeficiency virus
  18. Have gastrointestinal bleeding or documented active peptic ulcer within 4 weeks prior to Screening or entering a new study period
  19. Have chronic infection such as prior or active tuberculosis, active chicken pox or measles or absence of prior measles, mumps and rubella vaccine. Subjects with tuberculosis exposure or who live in, or travel to, high endemic areas should be assessed locally for tuberculosis before consideration for the study
  20. Immunosuppression or immunodeficiency disorder
  21. Have a history or presence of Crohn's disease, celiac disease, or other inflammatory disease of the gastrointestinal tract, including eosinophilic gastroenteritis
  22. Have current drug abuse in the opinion of the Investigator.
  23. Have current alcohol abuse in the opinion of the Investigator.
  24. Female subjects who are pregnant, breastfeeding, or planning to become pregnant during the study
  25. Sexually active females of childbearing potential who do not agree to follow highly effective contraceptive methods through the End of Study visit
  26. Have received an investigational product, as part of a clinical trial within 30 days (or 5 half-lives, whichever is longest) of Screening. Subjects who are currently participating in observational studies or enrolled in patient registries are allowed in this study
  27. Have participated in a prior study with investigational product APT-1011

Study & Design

Study Type
INTERVENTIONAL
Study Design
SEQUENTIAL
Arm && Interventions
GroupInterventionDescription
APT-1011APT-1011APT-1011 3 mg HS
APT-1011EsophagogastroduodenoscopyAPT-1011 3 mg HS
PlaceboPlacebo oral tabletHS
PlaceboEsophagogastroduodenoscopyHS
Primary Outcome Measures
NameTimeMethod
Week 12 histologic responder ratesWeek 12

To compare the Week 12 histologic responder rates (≤ 6 peak eosinophils \[eos\]/high power field \[HPF\]) for APT-1011 3 mg HS with that for placebo. HPF will be defined as a standard area of 235 square microns in a microscope with 40x lens (0.3 mm\^2) and 22 mm ocular

Mean change in number of dysphagia episodesWeek 0 to Week 12

To compare the mean change in number of dysphagia episodes from baseline to Week 12 for APT-1011 3 mg HS with that for placebo

Histologic responder rates at the end of the Randomized Withdrawal Phase (RWS)Week 12 to Week 52

To compare the histologic responder rates (≤ 6 peak eos/HPF) for APT-1011 responders randomized to continuing APT-1011 3 mg HS (maintenance) with responders randomized to placebo (withdrawal of APT-1011 3 mg HS) at the end of the RWS

Percentage subjects with complete symptomatic response at the end of the RWSWeek 0 to Week 52

Percentage of subjects with complete symptomatic response (i.e., no dysphagia episodes for the 14 consecutive days prior to the end of the randomized withdrawal phase) at the end of the randomized withdrawal phase, in the RWS APT-1011 3 mg HS arm versus placebo arm

Secondary Outcome Measures
NameTimeMethod
Change in EREFs from Week 0 to Week 12Week 0 to Week 12

To compare endoscopic appearance evaluated by the mean change from baseline to Week 12 in Eosinophilic Esophagitis Endoscopic Reference Score (EREFs) for APT-1011 3 mg HS with that for placebo.

Percentage of subjects with <1 peak eos/HPF at Week 12Week 12

To compare the percentage of subjects with \<1 peak eos/HPF at Week 12 for APT-1011 3 mg HS with that for placebo.

Mean change in PROSE Symptom Burden ScoreWeek 0 to Week 12

To compare the mean change from baseline to Week 12 in the day-level symptom burden utilizing the Patient Reported Outcomes Symptoms of EoE (PROSE) for APT-1011 3 mg HS with that for placebo.

Mean Change in PROSE Day-Level Difficulty SwallowingWeek 0 to Week 52

To compare the mean change in day-level difficulty swallowing using the Patient Reported Outcomes Symptoms of EoE (PROSE) from baseline to the end of randomized withdrawal phase, for APT-1011 responders randomized to continue APT-1011 3 mg HS with responders randomized to placebo (withdrawal of APT-1011 3 mg HS). Each symptom is rated on a numeric rating scale (NRS) with values ranging from 0 (not at all) to 10 (as bad as I can imagine).

Percentage of Subjects with <15 peak eos/HPFWeek 12

To compare the percentage of subjects with \<15 peak eos/HPF for APT-1011 3 mg HS with that for placebo.

Mean Number of Dysphagia-free DaysWeek 0 to Week 12

To compare the mean number of dysphagia-free days from baseline to Week 12 for APT-1011 3 mg HS with that for placebo

Mean Histologic Change from Baseline to Week 12Week 0 to Week 12

To compare mean histologic change from baseline to Week 12 for APT-1011 3 mg HS with that for placebo.

Mean Histologic ChangeWeek 0 to Week 52

To compare the mean change from baseline to the end of the RWS in peak eosinophil counts for APT-1011 responders randomized to APT-1011 3 mg HS with those randomized to placebo in the RWS.

Mean Change in PROSE Day-Level Symptom BurdenWeek 0 to Week 52

To compare the mean change in day-level symptom burden using the Patient Reported Outcomes Symptoms of EoE (PROSE) from baseline to the end of RWS, for APT-1011 responders randomized to continue APT-1011 3 mg HS with responders randomized to placebo (withdrawal of APT-1011 3 mg HS). Day-level symptom burden has values ranging from 0 (no symptoms) to 10 (symptoms are as bad as I can imagine).

Mean Change in Dysphagia EpisodesWeek 0 to Week 52

To compare mean change in number of dysphagia episodes from baseline to the end of RWS for APT-1011 responders randomized to continue APT-1011 3 mg HS with responders randomized to placebo (withdrawal of APT-1011 3 mg HS)

Mean Change in Number of Dysphagia EpisodesWeek 0 to Week 52

To compare mean change in number of dysphagia episodes from baseline at or prior to Week 52 (based on timing of \> 6 peak eos/HPF) for APT-1011 responders randomized to continue APT-1011 3 mg HS with those randomized to placebo (withdrawal of APT-1011 3 mg HS)

Mean Change in EREFs from Week 0 to Week 52Week 0 to Week 52

To compare endoscopic appearance evaluated by the mean change from baseline to the end of RWS, in Eosinophilic Esophagitis Endoscopic Reference Score (EREFs) for APT-1011 responders randomized to continue APT-1011 3 mg HS with responders randomized to placebo (withdrawal of APT-1011 3 mg HS). The EREF score has a range from 0-9, with 9 being worst result.

Mean Change in Dysphagia-Free DaysWeek 0 to Week 52

To compare the mean number of dysphagia-free days from baseline to the end of RWS, for APT-1011 responders randomized to continue APT-1011 3 mg HS with responders randomized to placebo (withdrawal of APT-1011 3 mg HS)

Trial Locations

Locations (56)

Gastro Center of Maryland

🇺🇸

Columbia, Maryland, United States

Henry Ford Health System

🇺🇸

Novi, Michigan, United States

DBC Research USA

🇺🇸

Pembroke Pines, Florida, United States

Long Island Gastrointestinal Research Group LLP

🇺🇸

Great Neck, New York, United States

Carolina Research

🇺🇸

Greenville, North Carolina, United States

Advanced Research Institute

🇺🇸

Ogden, Utah, United States

John Hunter Hospital

🇦🇺

New Lambton, New South Wales, Australia

St. Vincent's Hospital

🇦🇺

Fitzroy, Victoria, Australia

Hosital General de Tomelloso

🇪🇸

Tomelloso, Ciudad Real, Spain

Del Sol Research Management, LLC

🇺🇸

Tucson, Arizona, United States

Pinnacle Research Group, LLC

🇺🇸

Anniston, Alabama, United States

Preferred Research Partners Inc.

🇺🇸

Little Rock, Arkansas, United States

Arkansas Gastroenterology

🇺🇸

North Little Rock, Arkansas, United States

Camarillo Endoscopy Center

🇺🇸

Camarillo, California, United States

Hope Clinical Research

🇺🇸

Canoga Park, California, United States

Facey Medical Foundation

🇺🇸

Mission Hills, California, United States

Encore Borland Groover Clinical Research

🇺🇸

Jacksonville, Florida, United States

Bozeman Health GI Clinic

🇺🇸

Bozeman, Montana, United States

Clinical Research Professionals

🇺🇸

Chesterfield, Missouri, United States

Minnesota Gastroenterology, P.A.

🇺🇸

Plymouth, Minnesota, United States

Clinical Research Institute of Michigan LLC

🇺🇸

Chesterfield, Michigan, United States

West Michigan Clinical Research Center

🇺🇸

Wyoming, Michigan, United States

Verity Research, Inc.

🇺🇸

Fairfax, Virginia, United States

DHAT Research Institute

🇺🇸

Garland, Texas, United States

Gut P.C., dba; Digestive Health Specialists of the Southeast

🇺🇸

Dothan, Alabama, United States

East View Medical Research, LLC

🇺🇸

Mobile, Alabama, United States

United Medical Doctors

🇺🇸

Murrieta, California, United States

Asthma and Allergy Associates, PC

🇺🇸

Colorado Springs, Colorado, United States

Western States Clinical Research Inc.

🇺🇸

Wheat Ridge, Colorado, United States

Peak Gastroenterology Associates

🇺🇸

Colorado Springs, Colorado, United States

Western Connecticut Medical Group - Gastroenterology

🇺🇸

Danbury, Connecticut, United States

Fleming Island Center for Clinical Research

🇺🇸

Fleming Island, Florida, United States

Medical Research Center of Connecticut, LLC

🇺🇸

Hamden, Connecticut, United States

Nature Coast Clinical Research

🇺🇸

Inverness, Florida, United States

Summit Clinical Research

🇺🇸

Athens, Georgia, United States

University of Iowa Hospitals and Clinics

🇺🇸

Iowa City, Iowa, United States

MGG Group Co., Inc., Chevy Chase Clinical Research

🇺🇸

Chevy Chase, Maryland, United States

Great Lakes Gastroenterology Research, LLC

🇺🇸

Mentor, Ohio, United States

Perelman Center for Advanced Medicine

🇺🇸

Philadelphia, Pennsylvania, United States

Vital Prospects Clinical Research Institute, P.C.

🇺🇸

Tulsa, Oklahoma, United States

Northshore Gastroenterology Research, LLC

🇺🇸

Westlake, Ohio, United States

Digestive Disease Associates LTD

🇺🇸

Wyomissing, Pennsylvania, United States

Rapid City Medical Center LLP

🇺🇸

Rapid City, South Dakota, United States

Blue Ridge Medical Research

🇺🇸

Lynchburg, Virginia, United States

Swallow Clinic, St George Hospital

🇦🇺

Kogarah, New South Wales, Australia

St. Vincent's Hospital Sydney

🇦🇺

Darlinghurst, New South Wales, Australia

Lyell McEwin Hospital

🇦🇺

Elizabeth Vale, South Australia, Australia

Alfred Hospital

🇦🇺

Melbourne, Victoria, Australia

Hospital Universitario Ramón y Cajal (Madrid)

🇪🇸

Madrid, Spain

Medical Associates Research Group

🇺🇸

San Diego, California, United States

Endoscopic Research, Inc.

🇺🇸

Orlando, Florida, United States

Michigan Medicine, University of Michigan

🇺🇸

Ann Arbor, Michigan, United States

Mayo Clinic

🇺🇸

Rochester, Minnesota, United States

University of North Carolina Health Systems (UNC Hospital)

🇺🇸

Chapel Hill, North Carolina, United States

Consultants for Clinical Research

🇺🇸

Cincinnati, Ohio, United States

Bernstein Clinical Research Center, LLC

🇺🇸

Cincinnati, Ohio, United States

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