Effect of REST in the Alzheimer Disease Continuum

Completed

• Conditions: Alzheimer's Disease

• Registration Number: NCT03884023
• Lead Sponsor: Dongzhimen Hospital, Beijing

Brief Summary

The investigators assume that REST gene polymorphism affects REST protein concentration, and REST protein concentration in peripheral blood is related to cognitive function and hippocampus. In this current study, REST protein content and gene polymorphism will be obtained in peripheral blood in AD and normal control. The effect of REST gene polymorphism on REST protein concentration will be discovered.The relationship between REST protein concentration and cognitive function will be found, as well as the relationship between REST protein concentration and hippocampus.

Detailed Description

Repressor element silencing transcription factor(REST), also known as neuron-restrictive silencer factor (NRSF) is a zinc finger protein of 121 kD and belongs to the Gli-Kruppel family with transcriptional regulation.This will be a Case-Crossover research. Consecutive participants will be divided into Alzheimer's disease dementia group, mild cognitive impairment due to Alzheimer's disease group and normal control group according to inclusion criteria of this research. Peripheral blood samples will be retained to detect REST gene polymorphisms and protein concentrations. Participants perform neuropsychological tests to assess cognitive function. Medial temporal atrophy scale is used to describe hippocampus. Differences in the mean or proportions between these 3 groups will be checked by t test or test. The relationship between REST protein concentration and cognitive function will be estimated, as well as the relationship between REST protein concentration and hippocampus.

Study Timeline

• Study First Submitted: 2019-02-15
• Study First Submitted QC: 2019-03-19
• Study First Posted: 2019-03-21
• Study Start: 2019-06-28
• Primary Completion: 2020-11-30
• Study Completion: 2020-11-30
• Status Verified: 2022-03
• Last Update Submitted: 2022-03-22
• Last Update Posted: 2022-03-23

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 300

Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Rate of Gene polymorphism in rs2227902 and rs3796529.	baseline	Verifying the value of REST gene polymorphism as an early markers of Alzheimer's Disease
Concentration of REST protein	baseline	Verifying the value of REST protein detection as an early markers of Alzheimer's Disease

Trial Locations

Locations (1)

Dongzhimen Hospital,Beijing University of Chinese Medicine; 🇨🇳 Beijing, Beijing, China