Effect of an Exercise on Serum Uncoupling Protein-1 Level and the Role of Uncoupling Protein-1-3826 A/G Polymorphism

Not Applicable

Completed

• Conditions: Irisin; Uncoupling Protein-1; Polymorphism, Restriction Fragment Length
• Interventions: Other: Yo-Yo intermittent recovery test (level 1)

• Registration Number: NCT04156113
• Lead Sponsor: Eastern Mediterranean University

Brief Summary

This study aims to determine the effect of an acute maximal exercise on serum uncoupling protein-1, irisin, interleukin 6 levels, and on blood lipid and lipoprotein concentrations. This study also evaluates the role of uncoupling protein-1-3826 A/G polymorphism on this eventual effect.

Therefore, this study hypothesizes that:

H01: Sedentary people and athletes' serum uncoupling protein 1, irisin, and interleukine 6 levels are different before and after an acute maximal exercise.

H02: Sedentary people and athletes' basal serum uncoupling protein 1 levels are associated with uncoupling protein 1-3826 A/G polymorphism.

H03: Uncoupling protein 1-3826 A/G polymorphism has a modifying role in the effect of maximal exercise on serum uncoupling protein 1 levels.

H04: Sedentary people and athletes' basal serum lipid and lipoprotein levels are associated with uncoupling protein 1-3826 A/G polymorphism.

H05: Uncoupling protein 1-3826 A / G polymorphism has a modifying role in the effect of maximal exercise on serum lipid and lipoprotein levels.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2019-11-04
• Study First Submitted QC: 2019-11-06
• Study First Posted: 2019-11-07
• Study Start: 2019-11-11
• Status Verified: 2020-09
• Primary Completion: 2020-09-02
• Study Completion: 2020-09-07
• Last Update Submitted: 2020-09-07
• Last Update Posted: 2020-09-09

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 86

Inclusion Criteria

• Athletes group:

  • Must be basketball, or volleyball, or handball players.
  • Must be subjects who have been training regularly for at least 3 months.

• Sedentary group:

  * Must be subjects who have not been training regularly for at least 3 months.

• For both groups:

  • Healthy.
  • Non obese (body mass index < 30).
  • Aged between 18 and 35 years.

Exclusion Criteria

• For both groups:

  • Being regular consumer of cigarettes, alcohol, drugs and antioxidant substances.
  • Being obese (body mass index > 30).
  • Having an acute or chronic injury which is limiting physical performance.
  • Having a disease (anemia, infection...) detected from the biochemical analysis of the blood samples.
  • Being injured or sick during the study.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Athletes Group	Yo-Yo intermittent recovery test (level 1)	The athletes group will be composed of healthy, non obese (body mass index \< 30), male basketball, volleyball and handball players aged between 18 and 35 years who have been training regularly for at least 3 months. Participants must not be regular consumer of cigarettes, alcohol, drugs and antioxidant substances. Intervention: Yo-Yo intermittent recovery test (level 1) is administered.
Sedentary Group	Yo-Yo intermittent recovery test (level 1)	The sedentary group will be composed of healthy, non obese (body mass index \< 30), male aged between 18 and 35 years who have not been training regularly for at least 3 months. Participants must not be regular consumer of cigarettes, alcohol, drugs and antioxidant substances. Intervention: Yo-Yo intermittent recovery test (level 1) is administered.

Primary Outcome Measures

Name	Time	Method
Change from the baseline serum uncoupling protein 1 level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Uncoupling protein 1 analyzes were performed in the laboratory.
Change from the baseline serum irisin level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Irisin analyzes were performed in the laboratory.
Uncoupling protein-1-3826 A/G polymorphism determination	15 minutes before the Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1). Polymorphism was determined in the laboratory.

Secondary Outcome Measures

Name	Time	Method
Change from the baseline serum interleukin-6 level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Interleukine-6 analyzes were performed in the laboratory.
Change from the baseline serum nitric oxide level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Nitric oxide analyzes were performed in the laboratory.

Trial Locations

Locations (1)

Ege University Faculty of Sport Sciences; 🇹🇷 Izmir, Turkey