Molecular, Genomic, Cellular, and Functional Characterization of Blood Specimens From Former U.S. Service Members With Prior Burn Pit Exposure
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 1,000
- 主要终点
- Differential gene expression (RNA-seq normalized counts) between burn pit-exposed and unexposed cohorts
研究概览
简要总结
Project PHOENIX is an observational clinical research study designed to characterize molecular, genomic, cellular, and functional features in blood specimens from former U.S. Service Members with prior burn pit exposure and from matched unexposed controls. Participants will complete screening, informed consent, health and exposure questionnaires, and a one-time blood collection. Blood-derived specimens may undergo genomic, epigenomic, transcriptomic, proteomic, metabolomic, immunophenotyping, and cellular functional analyses. Participants may also agree to optional future re-contact for health updates and possible repeat blood collection. The goal is to identify biologic signatures associated with prior deployment-related burn pit exposure and to support future biomarker discovery and translational research in veteran health.
详细描述
Open-air burn pits used during military operations in Southwest Asia and other deployment settings have been associated with complex airborne exposures and long-term health concerns, including respiratory, cardiovascular, neurologic, immune, and oncologic sequelae. Prior studies have relied largely on self-report, clinical symptoms, or limited physiologic testing, and there remains a need for objective biologic markers of exposure-associated effects. Project PHOENIX is intended to generate a standardized, high-dimensional biospecimen and data resource that can support biomarker discovery, mechanistic modeling, and future translational research.
Participants will enroll remotely. Screening, consent, and questionnaire completion will occur electronically through a secure eConsent platform. After consent, participants will complete a baseline study visit that includes eligibility confirmation, collection of demographic and exposure history and venipuncture.
The exposed cohort will include former U.S. Service Members with prior deployment to locations with known burn pit operations. The control cohort will include comparable U.S. Service Members/Veterans without known burn pit deployment exposure. Planned analyses include between-group comparisons across multi-omic and cellular-function domains, assessment of exposure intensity/duration relationships, and development of integrated predictive models using statistical and machine-learning methods. Optional annual re-contact for up to 5 years may be used to update health status and obtain additional blood specimens.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Age 18 years or older at the time of consent
- •Former or current U.S. Service Member, including Active Duty, Reserve, National Guard, or Veteran status
- •For exposed cohort: prior deployment to a location with known burn pit operations, based on participant report and available confirmation when feasible
- •For control cohort: no known deployment to burn pit locations
- •Able and willing to provide informed consent and HIPAA authorization
- •Willing to complete study questionnaires and donate venous blood sample
- •Able to complete study procedures in English or with approved translation support
- •Available for one baseline visit and optional future re-contact, if applicable
排除标准
- •Active serious illness or infection that, in the investigator's judgment, would compromise participation or specimen integrity
- •Receipt of systemic chemotherapy, immunotherapy, or radiation therapy within the past 6 months
- •High-dose systemic immunosuppression, defined as more than 20 mg prednisone equivalent daily for more than 14 days within the past 3 months
- •Pregnant or currently breast-feeding
- •Hemoglobin less than 10 g/dL, known bleeding disorder, or platelet count less than 100 × 10^9/L, if known
- •Severe psychiatric illness or other condition that impairs ability to provide informed consent
- •Prisoner or institutionalized individual
- •Prior participation in this or a related Project PHOENIX protocol
- •Any other condition that, in the investigator's judgment, may increase risk or interfere with study conduct or data integrity
研究组 & 干预措施
Prior Burn Pit Exposure
Former U.S. Service Members with prior deployment to locations with known burn pit operations, based on self-report and available service/registry confirmation when feasible. Participants will complete study questionnaires, brief health assessments, and one-time blood collection for multi-omic, cellular, and functional analyses. Optional future re-contact may occur.
干预措施: Prior Burn Pit Exposure (Other)
Unexposed Control
Former U.S. Service Members/Veterans without known deployment-related burn pit exposure, frequency-matched at the group level to the exposed cohort on selected demographic/service characteristics. Participants will complete study questionnaires, brief health assessments, and one-time blood collection for comparative analyses. Optional future re-contact may occur.
干预措施: No Known Burn Pit Exposure (Other)
结局指标
主要结局
Differential gene expression (RNA-seq normalized counts) between burn pit-exposed and unexposed cohorts
时间窗: Baseline
Comparison of whole blood transcriptomic profiles using RNA sequencing. Differential gene expression will be quantified using normalized read counts and modeled as log2 fold-change between cohorts.
次要结局
- Association of exposure intensity and duration with biologic measures(Baseline)