The Acute Effects of Interesterification of Commercially Used Fats on Postprandial Fat Metabolism
- Conditions
- Healthy AdultsPostmenopausal Women
- Interventions
- Dietary Supplement: Un-interesterified blend of palm kernal and plam stearinDietary Supplement: Interesterified blend of palm kernal and plam stearinDietary Supplement: Rapeseed oil
- Registration Number
- NCT03191513
- Lead Sponsor
- King's College London
- Brief Summary
The purpose of this study is to investigate whether there are differences in postprandial metabolic indices following interesterified fats used commercially versus the corresponding un-interesterified blend.
- Detailed Description
Aim: The current study aims to investigate the acute effects of commercially relevant interesterified 'hardstock' versus the corresponding un-interesterified blend on 8 h postprandial fat metabolism.
Hypothesis: Interesterification of a palm kernel and palm stearin fat blend, to produce a fat with a higher proportion of palmitic acid in the middle position of the triglyceride (TAG; but the same fatty acid composition), will alter postprandial lipid metabolism.
Subjects: Participants will include 24 healthy male and female (postmenopausal) volunteers aged between the ages of 45 and 75 years (since during this age metabolic changes start to take place).
Power calculation: Based on previous studies carried out by our group at King's College London, a sample size of 10 males and 10 females is required to detect a (clinically relevant) difference (for males and females separately) between means of 0.3 mmol/L maximal difference in 8 h plasma TAG concentration (standard deviation 0.33 mmol/L, significance level (alpha) of 0.05 and 80% power). 12 males and 12 females will be recruited to allow for a 20% drop out rate.
Expected value:The study will provide novel information on the acute effects of commercially relevant processed fats on postprandial lipaemia.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 24
- Males and females aged 45-75 years
- Healthy (free of diagnosed diseases listed in exclusion criteria)
- Able to understand the information sheet and willing to comply with study protocol
- Able to give informed consent
- Medical history of myocardial infarction, angina, thrombosis, stroke, cancer, liver or bowel disease or diabetes
- Body mass index < 20 kg/m2 or > 35 kg/m2
- Plasma cholesterol ≥7.5 mmol/L
- Plasma triacylglycerol > 3 mmol/L
- Plasma glucose > 7 mmol/L
- Blood pressure ≥140/90 mmHg
- Current use of antihypertensive or lipid lowering medications
- Premenopausal (for women)
- Alcohol intake exceeding a moderate intake (> 28 units per week)
- Current cigarette smoker (or quit withint the last 6 months)
- ≥ 20% 10-year risk of CVD as calculated using a risk calculator
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Un-interesterified Un-interesterified blend of palm kernal and plam stearin Un-interesterified blend of palm kernal and plam stearin. 50g fat. Interesterified Interesterified blend of palm kernal and plam stearin Interesterified blend of palm kernal and plam stearin. 50g fat. Control Rapeseed oil Rapeseed oil. 50g fat.
- Primary Outcome Measures
Name Time Method Area under plasma TAG concentration/ time curve Up to 8 hours Postprandial area under plasma TAG concentration/ time curve
- Secondary Outcome Measures
Name Time Method Positional composition retention (chylomicron) Up to 6 hours Postprandial chylomicron TAG concentration and composition, TAG sn-2 fatty acid composition, total protein, apoB100 and apoB48
Postprandial lipaemic response Up to 8 hours Postprandial plamsa total fatty acid composition and non esterifed fatty acid
Trial Locations
- Locations (1)
King's College London
🇬🇧London, United Kingdom