The Role of Dehydroepiandrosterone in the Maintenance of Mitochondrial Quality of Cumulus Cells in Poor Ovarian Responders
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 66
- 试验地点
- 3
- 主要终点
- mitochondria related genes expression
研究概览
简要总结
To investigate whether the DHEA supplementation could improve mitochondrial quality in poor ovarian responders
详细描述
Women who underwent in vitro fertilization (IVF) treatment participated, including normal ovarian responders (NORs) and poor ovarian responders (PORs). PORs were assigned to receive DHEA supplementation or not before the IVF cycle. For all patients, cumulus cells (CCs) were obtained after oocyte retrieval. In the CCs, mRNA expression of mitochondria-related genes was measured. To compare the mRNA expression of mitochondria-related genes in the CCs among the three groups.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 25 Years 至 45 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •Poor ovarian responders met the Bologna criteria, having at least two of the three following features: (1) advanced maternal age (≥ 40 years) or any other risk factor for POR, (2) a previous POR (≤ 3 oocytes with a conventional stimulation protocol), and (3) an abnormal ovarian reserve test. An abnormal ovarian reserve test was defined as antral follicle counts (AFC) < 5 or anti-Müllerian hormone (AMH) < 1 ng/mL in this study.
- •Normal ovarian responders met the following criteria: (1) AFCs ≥ 5 or AMH ≥ 1 ng/mL and (2) the number of retrieved oocytes was between 5 and 15.
排除标准
- •previous oophorectomy
- •exposure to cytotoxic or pelvic irradiation for malignancy
- •positive screening for recurrent pregnancy loss (chromosome mapping, antinuclear antibodies, extractable nuclear antigens, antiphospholipid antibodies, thrombophilic screening)
- •any other sensitizing or ovarian stimulating therapy during the previous 3 months
结局指标
主要结局
mitochondria related genes expression
时间窗: through study completion, an average of 1 year
cumulus cells genes expression
次要结局
- oocytes(through study completion, an average of 1 year)
- pregnancy rate(pregnancy will be confirmed 4 weeks after embryo transfer)
- embryos(numbers will be confirmed 3 days after fertilization)
研究者
Li-Te Lin
Visiting Staff, Department of Obstetrics and Gynecology, Principal Investigator, Clinical Lecturer
Kaohsiung Veterans General Hospital.