Impact of fat co-ingestion with protein on the post-prandial anabolic response in elderly me
Completed
- Conditions
- age-related muscle losssarcopenia1003700810028302
- Registration Number
- NL-OMON37092
- Lead Sponsor
- niversiteit Maastricht
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 32
Inclusion Criteria
* Healthy males
* Age between 55 and 85
* BMI < 30 kg/m2
Exclusion Criteria
* Glucose intolerance
* Milk and/or fat intolerance
* Smoking
* Diagnosed GI tract diseases
* Arthritic conditions
* A history of neuromuscular problems
* Any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
* Use of anticoagulants
* Participation in exercise program
* Hypertention, high blood pressure above 140/90 mmHg
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>The main study endpoint is muscle protein synthesis (MPS) rate. In order to<br /><br>determine the MPS, the following parameters will be measured in blood and<br /><br>muscle tissue:<br /><br>* Plasma phenylalanine concentration (expressed as *mol/L)<br /><br>* Plasma enrichment of L-[ ring-2H5]-phenylalanine and L-[1-13C]-phenylalanine<br /><br>(expressed as mole percent excess, MPE)<br /><br>* Muscle protein bound enrichment of L-[ ring-2H5]-phenylalanine and<br /><br>L-[1-13C]-phenylalanine (expressed as MPE)<br /><br>* L-[ ring-2H5]phenylalanine and L-[1-13C]-phenylalanine enrichment of the<br /><br>muscle free amino acid pool (expressed as MPE)</p><br>
- Secondary Outcome Measures
Name Time Method <p>Secondary endpoints include whole-body protein turnover, and protein digestion<br /><br>and absorption kinetics. The following parameters will be calculated:<br /><br>* Total rate of phenylalanine appearance and disappearance (= protein turnover)<br /><br>* Exogenous phenylalanine rate of appearance<br /><br>* Endogenous phenylalanine rate of appearance (=protein breakdown)<br /><br>* Plasma availability of phenylalanine</p><br>