Investigation of Novel Targetable Kinases in Steroid-refractory Acute Graft-versus-host Disease
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 主要终点
- Identification of differentially regulated kinases
研究概览
简要总结
In this study, the investigators aim to identify novel targetable kinases in SR-a GvHD patient samples and investigate their role in different immune cell subtypes.
详细描述
Corticosteroids are the first-line treatment of a GvHD, however many patients do not respond to corticosteroids. Patients with steroid-refractory a GvHD (SR-a GVHD) have a low 1-year survival rate. In this study, the investigators aim to identify and validate novel targetable kinases in SR-a GvHD patient peripheral blood mononuclear cells (PBMCs) by using a kinase-specific proteomic approach, and thereafter to investigate the role of the identified kinases in different immune cell subtypes by analyzing biopsies taken from SR- a GvHD patients.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •allo-transplanted
- •confirmed diagnosis of a GvHD
- •age ≥ 18 years
- •peripheral blood samples and biopsies available
- •written informed consent
- •ability to understand the nature of the study and the study-related procedures and to comply with them
排除标准
- •age < 18 years
- •lack of informed consent
- •patients that cannot be classified in one of the 2 groups (steroid-refractory, steroid-responsive)
结局指标
主要结局
Identification of differentially regulated kinases
时间窗: 3 years
Bead-based Kinase Assay enrichment (Knet beads) of PBMC lysates
次要结局
- Analysis of protein expression(3 years)
- Analysis of immune cell subtype(3 years)
研究者
Robert Zeiser
Director of Division of Tumor Immunology
University of Freiburg