The Effect of Low Oxygen Tension on the Early Development of Human Embryo in IVF/ICSI
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- embryo quality
研究概览
简要总结
To investigate the effect of low oxygen tension during in vitro culture in terms of embryo quality and outcomes of pregnancy in vitro fertilization/ Intracytoplasmic sperm injection
详细描述
The oocytes will be divided into two parts(two groups) form one patient. The two groups of oocytes will be cultured seperately in the incubator with the concentrations of 5% CO2,6%O2,89% N2(low oxygen concentration) verse 6% CO2,20% O2,74% N2(in atmosphere).
A series of observational measurement(embryo quality and outcomes of pregnancy) will be performed.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •Fresh cycles of in vitro fertilization/intracytoplasmic sperm injection and emrbyo transfer (IVF/ICSI-ET)
- •≥ 10 oocytes after oocyte retrieval
排除标准
- •≥ 3 transfer or oocyte retrieval cycles
- •PGD/PGS cycles
- •half-ICSI/rescure ICSI
- •fluid in the uterine cavity, abnormal endometrium, uterine abnormalities,pelvic inflammation
- •acute maternal problems.
- •One or both spouses have an abnormal karyotype (including polymorphism)
结局指标
主要结局
embryo quality
时间窗: up to 6 days after fertilization
Embryo morphology was assessed on day 2, day 3 day 4 day 5 day and 6 by considering the number of blastomeres, symmetry and granularity of blastomeres, type and percentage of fragmentation, presence of multinucleated blastomeres, and degree of compaction. Gardner's blastocyst scoring system is used for blastocyst assessment
次要结局
未报告次要终点
研究者
Shi Wenhao
Assisted Reproductive Technology Center
Shanxi Provincial Maternity and Children's Hospital