Sex-difference in Response to Resistance Exercise and Training
试验速览
- 阶段
- 不适用
- 状态
- Enrolling By Invitation
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- Sex differences in protein expression of muscle mass regulation pathways assessed by western blot
研究概览
简要总结
Sex-Difference in Response to Resistance Exercise and Training
Given the importance of muscular strength for athletic performance as well as for daily activities in the general population-including clinical populations-and the lack of data in both men and women, the aim of this study is to compare neuromuscular, intramuscular, and systemic adaptations in men and women in response to both acute and chronic resistance exercise.
Specifically, the investigators will test the hypotheses that:
the neuromuscular response to resistance exercise, including corticospinal excitability and motor unit activation patterns, differs between men and women; the hormonal response and exosome release following resistance exercise are reduced in women compared to men.
Participants (n = 15 per group) will follow a supervised resistance training program for 8 weeks. Neuromuscular function of the knee extensor muscles, body composition, intramuscular adaptations, as well as blood profiles (hormonal and metabolic) and exosomes will be assessed before and after a single resistance exercise session and after the training period. This protocol will allow determination of both the acute and long-term effects of resistance exercise.
Women's participation in sports is increasing, yet research in this area remains very limited. This project aims to better understand the mechanisms by which resistance training-a non-pharmacological and low-cost strategy-may induce different adaptations in men and women, and, where appropriate, to contribute to the development of sex-specific recommendations to maximize the health benefits of resistance training.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Men and women aged between 18 and 40 years;
- •eumenorrheic women or under contraceptive (hormonal and non-hormonal).
排除标准
- •Regular resistance exercise (≥2x/week);
- •Any contraindication to exercise;
- •Protein/creatine supplement intake;
- •Pregnancy;
- •Epilepsy;
- •Head implant.
结局指标
主要结局
Sex differences in protein expression of muscle mass regulation pathways assessed by western blot
时间窗: Throughout the entire study, during approximately 11 months
Assessment of protein of expression muscle mass regulation pathways after acute exercise and chronic exercise using western blot (ratio of target protein to reference protein)
Sex differences in mRNA levels in muscle mass regulation pathways assessed by RT-qPCR
时间窗: Throughout the entire study, during approximately 11 months
Assessment of mRNA levels of muscle mass regulation pathways after acute exercise and chronic exercise usin RT-qPCR (fold change).
Sex differences in metabolic status assessed by blood biomarkers
时间窗: Throughout the entire study, during approximately 11 months
Assessment of metabolic blood parameters before and after acute exercise and chronic resistance training.
Sex differences in hormonal status assessed by blood biomarkers
时间窗: Throughout the entire study, during approximately 11 months
Assessment of hormonal blood parameters before and after acute exercise and chronic resistance training.
Sex differences in extracellular vesicles regulation assessed by nanoparticle tracking analysis
时间窗: Throughout the entire study, during approximately 11 months
Assessment of the concentration and size of circulating extracellular vesicles after acute exercise and chronic exercise using nanoparticle tracking analysis.
Sex differences in extracellular vesicles regulation assessed by proteomic analysis
时间窗: Throughout the entire study, during approximately 11 months
Assessment of protein expression of circulating extracellular vesicles after acute exercise and chronic exercise using proteomic analysis
次要结局
- Sex differences in body composition assessed by dual-energy X-ray absorptiometry (DXA)(Throughout the entire study, during approximately 11 months)
- Sex differences in muscle thickness of the vastus lateralis assessed by ultrasonography(Throughout the entire study, during approximately 11 months)
- Sex differences in strength evolution assessed by one-repetition maximum (1-RM)(Throughout the entire study, during approximately 11 months)