Caffeine Supplementation, Resistance Training and Blood Antioxidant Status

Not Applicable

Completed

• Conditions: Caffeine
• Interventions: Dietary Supplement: Caffeine supplementation; Dietary Supplement: Placebo treatment

• Registration Number: NCT05230303
• Lead Sponsor: The Jerzy Kukuczka Academy of Physical Education in Katowice

Brief Summary

The purpose of this study is to verify the effect of acute caffeine intake on resistance training volume and blood antioxidant status, in a randomised, double-blind, placebo-controlled crossover trial.

Detailed Description

Caffeine is one of the most commonly consumed psychoactive substances in the world. Several previous studies confirmed that caffeine improves several aspects of exercise performance, including resistance training outcomes. Interestingly, caffeine and its catabolic products, theobromine and xanthine, exhibit antioxidant and prooxidant properties. Additionally, resistance training promotes the generation of reactive oxygen species, which are important for the cellular adaptation process.

Hovewer, to the best of our knowledge, there is no information as to how caffeine combined with resistance training affects blood antioxidant status. Therefore, the aim of this study was to examine acute effects of caffeine supplementation on resistance training volume and blood antioxidant status in resistance trained men.

Study Timeline

• Study Start: 2022-01-12
• Study First Submitted: 2022-01-14
• Study First Submitted QC: 2022-01-28
• Study First Posted: 2022-02-08
• Primary Completion: 2023-01-31
• Study Completion: 2023-01-31
• Status Verified: 2023-03
• Last Update Submitted: 2023-03-08
• Last Update Posted: 2023-03-10

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 15

Inclusion Criteria

• written consent to participate,
• medical permission by physician
• "resistance-trained," defined as having a minimum of two years of resistance training experience
• minimum of 2 workout sessions per week in the last 6 months

Exclusion Criteria

• neuromuscular or musculoskeletal disorders
• current injury
• using any medications, dietary supplements or ergogenic aids which could potentially affect the study outcomes
• a positive smoking status
• potential allergy to caffeine

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Caffeine supplementation	Caffeine supplementation	Group taking 3 mg/kg of caffeine
Placebo treatment	Placebo treatment	Group taking placebo

Primary Outcome Measures

Name	Time	Method
Changes in markers of liver function	Day 1, Day 2	Serum will be obtained for activity of ASPAT (aspartate aminotransferase), ALAT (alanine aminotransferase) and gamma glutamyltransferase (GGT)
Change the levels of oxidative stress markers	Day 1, Day 2	Serum will be obtained for TOS/TOC (total oxidative stress), MDA (malondialdehyde), oncentration of TAS (total antioxidant status), activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx); concentartion of GSH (reduced glutathione) and UA (uric acid)
Changes in markers of muscle damage	Day 1, Day 2	Serum will be obtained for activity of creatine kinase (CK) and lactate dehydrogenase (LDH), concentration of myoglobin (Mb)
Changes in Inflammation markers	Day 1, Day 2	Serum will be obtained for level of interleukin 6, TNF alfa (tumor matrix factor), CRP (C-reactive protein)

Secondary Outcome Measures

Name	Time	Method
Changes in muscle soreness	prior to exercise, and 24, 48, 72, 96 and 120 hours post exercise.
Changes in rate of perceived exertion after resistance training	Immediately after exercise protocol during caffeine and placebo treatment
Changes in heart rate during resistance training	During caffeine and placebo treatment
Changes in resistance training volume	During caffeine and placebo treatment

Trial Locations

Locations (1)

The Jerzy Kukuczka Academy of Physical Education; 🇵🇱 Katowice, Poland