Microbiological and pathological analysis of explanted Bone Flaps with different cryopreservation after decompressive hemicraniectomy

• Conditions: S06; Intracranial injury; I63; Cerebral infarction

• Registration Number: DRKS00023283
• Lead Sponsor: Director, Department of Neurosurgery ,Katholisches Klinikum Lünen/Werne (KKLW)

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Study Completion: 2020-10-04
• Study Start: 2020-10-26
• Last Update Posted: 19 August 2024

Recruitment & Eligibility

• Status: Complete
• Sex: All
• Target Recruitment: 18

Inclusion Criteria

The patients, who have been in the
Neurosurgical Clinic at the Catholic
Klinikum Lünen / Werne Bone flaps explanted, which in the context of an Exitus letalis were not re-implanted.

Exclusion Criteria

none

Study & Design

• Study Type: observational
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
The comparison of the microbiological status between explanted Bone flaps (subgroup A vs. subgroup B) using microbiological culture.<br><br>The comparison of the bony microarchitecture of the explanted Bone flaps (subgroup A vs. subgroup B) using microscopy and the expression analysis of biomarkers of bone viability (parathyroid hormone type I receptor (PTH1R), osteoprotegerin (OPG), receptor of NFkB (RANKL), osteocalcin, C-terminal telopeptide) using immunohistochemistry (Subgroup A vs. subgroup B) in the explanted cranial bone (cortex and spongiosa)

Secondary Outcome Measures

Name	Time	Method
The comparison of the infection potential after contamination of the explanted Bone flaps and the CAD with specific bacterial strains, i.e. whether pathogens grow at different speeds in the various subgroups. (Subgroup A vs. Subgroup B vs. Subgroup C)