Influence of the Glycemic and Ponderal Status on Tissues Gene Expression (Biological Tissue Collection)
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Obesity
- Sponsor
- University Hospital, Lille
- Enrollment
- 20000
- Locations
- 1
- Primary Endpoint
- Study the influence of phenotypic characteristics on gene expression of tissues involved in glucose metabolism
- Status
- Recruiting
- Last Updated
- 4 months ago
Overview
Brief Summary
Type 2 diabetes and obesity are both multifactorial diseases resulting from gene-environment interactions. However, this interaction, as well as the specific effect of each polymorphism, remains poorly understood.
We now proposed a prospective cohort study to improve our understanding of the influence of phenotypic characteristics on gene expression in tissues involved in glucose and/or lipid metabolism by collecting different biological samples.
Detailed Description
Type 2 diabetes (T2D) is a disease commonly associated with obesity, which is an important risk factor for this condition. More than 80% of the diabetic subjects are obese. By analogy with the metabolic syndrome, the close association between obesity and T2D justifies the recognition of a new disease entity named by the neologism "diabesity". This study will examine the contribution of different genetic variants on "diabesity" development, by integrating multiple genomics approaches (linkage analysis on whole genome, transcriptomics and bioinformatics) and analysis of biological pathways in relevant animals models and humans.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Age between 18 and 65 years
- •Indication of abdominal surgery requiring a laparotomy or laparoscopy for bariatric surgery, cholecystectomy, or parietal surgical
- •Phenotype corresponding to one of the following four cases :
- •Body Mass Index ≥ 35 kg/m2 and diabetes defined by a fasting blood glucose ≥ 7 mmol/l and/or ≥ to 11.1 mmol/l, 120 minutes after ingestion of glucose (hyperglycemia caused by oral route)
- •Body Mass Index ≥ 35 kg/m2 with intolerance glucose defined by a fasting blood glucose\> 6 mmol/L and \<7 mmol/l and/or\> 7.8 mmol/l and \<11.1 mmol/l , 120 minutes after ingestion of glucose (hyperglycemia caused by oral route)
- •Body Mass Index ≥ 35 kg/m2 without diabetes defined by a blood glucose ≤ 6 mmol/L and / or ≤ 7.8 mmol/l, 120 minutes after ingestion of glucose (hyperglycemia caused by oral route)
- •Body Mass Index \<27 kg/m2 without diabetes defined by a blood glucose ≤ 6 mmol/L and / or ≤ 7.8 mmol/l, 120 minutes after ingestion of glucose (hyperglycemia caused by oral route)
- •27 \<Body Mass Index \<35 kg/m2 without diabetes defined by a blood glucose ≤ 6 mmol/L and / or ≤ 7.8 mmol/l, 120 minutes after ingestion of glucose (hyperglycemia caused by oral route)
Exclusion Criteria
- •unable to receive clear information
- •refusal to sign the consent form
- •pathology associated judged by the surgeon, may increase the risk of adverse events related to sampling tissue
Outcomes
Primary Outcomes
Study the influence of phenotypic characteristics on gene expression of tissues involved in glucose metabolism
Time Frame: Baseline
Study the correlation between the glycemic status (fasting glucose and / or after ingestion of glucose) adjusted to the presence or absence of obesity (Body Mass Index) and gene expression in tissues involved in glucose metabolism before bariatric surgery
Secondary Outcomes
- Prospective assessment of clinical ans biological features before and after bariatric surgery(5 years)
- Genotype-Phenotype correlation(Baseline)
- Correlation between gene expression and tissue insulin resistance index (HOMA2) (The Homeostasis Model Assessment)(5 years)
- Prospective assessment of clinical and biological features before and after bariatric surgery(2 years)