Determination of the Antibacterial effect of Clearfil Protect Bond in-vivo – Identification of Polybacterial 16S rRNA genes in caries samples by T-RFLP and direct sequencing
Not Applicable
- Conditions
- K02.1K02.9Caries of dentineDental caries, unspecified
- Registration Number
- DRKS00011532
- Lead Sponsor
- niversitätsklinikum Leipzig
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Complete
- Sex
- All
- Target Recruitment
- 10
Inclusion Criteria
•primary caries lesions at 2 (or more) molars or premolars with indication for a stepwise or partial caries removal (regardless of the present trial). Two of the lesions will be included into the study.
-clinical picture: ICDAS 4, 5 or 6
-extension in the X-ray picture: R4 (inner half of the dentin)
-no pulpal symptoms of the teeth to be included into the study
-maintained sensibility of the study teeth
•informed consent
Exclusion Criteria
• known history of intolerance towards one of the adhesive systems or one of their ingredients
• unability of determining study outcome and probable consequences (e.g. because of alcoholism)
• refusal to give consent
• pregnancy, breastfeeding
• inadequate adherence
Study & Design
- Study Type
- interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Determination of the antibacterial effect of Clearfil Protect Bond on residual caries compared to Clearfil SE Bond 2. Sampling of carious material at the first visit and at a reentry after eight weeks. Samples are analyzed by microbiological and molecular biological methods (as described in the summary). Changes in microbiological composition are determined quantitatively (qPCR) as well as qualitatively.
- Secondary Outcome Measures
Name Time Method Determination of the bacterial composition of progressed dentin caries lesions before and after partial caries removal and application of different therapy strategies