Oxidative Stress as an Acute Exercise-induced Mechanism of Stem and Progenitor Cell Mobilization

Not Applicable

Completed

• Conditions: Healthy
• Interventions: Dietary Supplement: Antioxidative supplementation

• Registration Number: NCT03747913
• Lead Sponsor: Swiss Federal Institute of Technology

Brief Summary

It is already known from literature that exercise mobilizes stem and progenitor cells into the peripheral blood. However, the exact mechanisms thereof remain to be fully elucidated.

The investigators hypothesize that exercise-induced oxidative stress could be at least one of the responsible mechanisms and therefore want to study the exercise-induced stem and progenitor cell mobilization in a group of healthy young men when they exercise with, compared to when they exercise without antioxidative supplementation.

The primary outcome is numbers of stem and progenitor cells in the peripheral blood after an acute bout of exercise. As a secondary outcome, numbers of apoptotic mature and immature cells in the blood will be analysed.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2018-11-12
• Study First Submitted QC: 2018-11-16
• Study First Posted: 2018-11-20
• Study Start: 2019-05-02
• Primary Completion: 2019-10-21
• Study Completion: 2019-10-31
• Status Verified: 2019-12
• Last Update Submitted: 2019-12-13
• Last Update Posted: 2019-12-16

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 18

Inclusion Criteria

• Non-smoking
• Healthy
• Normal Body-Mass-Index (BMI): 18.5-24.9 kg∙m-2
• Active (fulfill World Health Organization-guidelines for recommended levels of physical activity for adults aged 18 - 64 years)
• Willing to adhere to the study rules

Exclusion Criteria

• Known or suspected non-compliance, drug or alcohol abuse
• Inability to follow the procedures of the study, e.g. due to language problems, psychological disorders etc. of the participant
• Previous enrolment into the current study
• Enrolment of the investigator, his/her family members, employees and other dependent persons
• Regular intake of medication
• Regular intake of antioxidant supplementations

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Antioxidative Supplementation	Antioxidative supplementation	Each participant conducts two identical cycling tests, first without and a week later with antioxidative supplementation

Primary Outcome Measures

Name	Time	Method
Changes in exercise-dependently mobilized stem and progenitor cell numbers in the peripheral blood with antioxidative supplementation	On visit 2 after the exercise test without supplementation and then again on visit 3 after the exercise test with prior supplementation (1 week)	Numbers of hematopoietic, endothelial and mesenchymal stem and progenitor cells and mature endothelial cells in the peripheral blood will be measured after an acute bout of exercise first without and then with prior antioxidative supplementation.

Secondary Outcome Measures

Name	Time	Method
Changes in numbers of exercise-induced apoptotic cells and oxidative stress markers in the peripheral blood with antioxidative supplementation	On visit 2 after the exercise test without supplementation and then again on visit 3 after the exercise test with prior supplementation (1 week)	Numbers of apoptotic mononuclear cells, apoptotic hematopoietic, endothelial and mesenchymal stem and progenitor cells and apoptotic mature endothelial cells, as well as in oxidative stress markers in the peripheral blood will be measured after an acute bout of exercise first without and then with prior antioxidative supplementation.

Trial Locations

Locations (1)

Exercise Physiology Lab, Institute of Human Movement Sciences and Sport, ETH Zurich; 🇨🇭 Zürich, Zurich, Switzerland