Effect of high- and low-fructose diets on intestinal fat productio
- Conditions
- De novo lipogenesis (DNL) and post-prandial TAG levels, which are an established independent risk factor for cardiovascular disease.Nutritional, Metabolic, Endocrine
- Registration Number
- ISRCTN93148765
- Lead Sponsor
- niversity of Surrey
- Brief Summary
2020 results in https://pubmed.ncbi.nlm.nih.gov/32549314/ (added 16/07/2020)
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Male
- Target Recruitment
- 6
1. Gender: Male
2. Ethnicity: Caucasian
3. Age: 18 – 50 years
4. BMI: 25 – 32 kg/m²
5. < 3 sessions of aerobic exercise per week
6. Weight stable for the previous 3 months (± 2.5 kg)
7. Normal blood haemoglobin content (13.5-17.5 g/dL)
8. Blood pressure below 160/100 mmHg (systolic/diastolic)
1. Fasting plasma glucose > 7 mmol/L
2. Fasting plasma TAG > 4 mmol/L
3. Smoker
4. Type 1 or type 2 diabetes
5. Prescribed lipid-lowering medications
6. Endocrinological, cardiovascular, gastrointestinal and liver diseases or renal impairment
7. History of eating disorders, nausea or vomiting
8. Alcohol intake > 32 g/day or history of drug abuse
9. Relevant food allergies or intolerances e.g. fructose
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method CM-TAG production rate and clearance rate, measured at using intravenous stable isotope labelled glycerol (2H5-glycerol) administered during the post-prandial steady-state during each study visit
- Secondary Outcome Measures
Name Time Method <br> Measured during by hourly blood sampling at each post-prandial study visit:<br> 1. VLDL-TAG production rate and clearance rate (measure using stable isotope 2H5-glycerol infusion)<br> 2. Rate of intestinal (CM) and hepatic (VLDL) de novo lipogenesis (measured using oral 2H2O)<br> 3. Post-prandial TAG concentration (measured via enzymatic assay)<br>