Identification and characterization of pruritus pathways in chronic skin inflammation: microbes as exogenous drivers of itch

Recruiting

Conditions: L20
Atopic dermatitis

Registration Number: DRKS00025171

Lead Sponsor: niversitätsklinik für Dermatologie und Allergologie, Klinikum Oldenburg

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Recruiting

Sex: All

Target Recruitment: 60

Inclusion Criteria

Patients with chronic pruritus (due to lichen planus, psoriasis and atopic dermatitis); minimum age 18 years

Exclusion Criteria

Patients who cannot complete the questionnaires due to physical or psychiatric diseases or an insufficient understanding of the German language. Patients taking antipruritic drugs. Patients with an infection at the potential biopsy area. Patients who received anti-inflammatory therapy within the last two weeks and pregnant or breast-feeding women are excluded from the study.

Study & Design

Study Type: observational

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Microbiome characterization of skin at each time point of intervention (d0, 1, 7, 30); expression profiles of pruritic mediators (immunohistochemical and immunofluorescence staining, ELISA, Western blots, Luminex).<br>Gene expression profiles (qPCR, RNA sequencing), <br>Patient questionnaires (d0, 1, 7, 30): NeuroDerm questionnaire, 5PLQ, SF12, Beck Depression Inventory (BDI), STAI-T (State-Trait Anxiety Inventory), SCQ (Self-Administered Comorbidity Questionnaire), BPI (Brief Pain Inventory), Pain Catastrophizing Scale, NPSI (Neuropathich pain symptom inventory).

Secondary Outcome Measures

Name	Time	Method
Findings of this study will increase our understanding of molecular and cellular pathways inducing pruritus with an emphasis on understanding the role of IL-31RA signaling, will define and validate biomarkers for chronic pruritus and potentially identify disease-specific biomarkers, will characterize known therapeutic targets for the treatment of chronic pruritus and identify future therapeutic perspectives and will unravel the role of microbes as exogenous triggers of itch.