Exercise-induced Changes in DNA-methylation

Not Applicable

Terminated

• Conditions: Healthy
• Interventions: Behavioral: High-intensity interval training; Behavioral: Moderate training

• Registration Number: NCT01931072
• Lead Sponsor: Norwegian University of Science and Technology

Brief Summary

The purpose of the study was to investigate whether regular moderate- or high-intensity interval training can change the DNA methylation in a long-lasting state, and whether these changes affect the gene expression. In addition, the investigators will determine whether the intensity of the training could be an affecting factor of the possible DNA-methylation change as well. The null hypothesis was that no such exercise-induced changes occur.

Detailed Description

Not available

Study Timeline

• Study Start: 2012-08
• Primary Completion: 2012-12
• Study First Submitted: 2013-08-26
• Study First Submitted QC: 2013-08-28
• Study First Posted: 2013-08-29
• Study Completion: 2013-12
• Status Verified: 2019-05
• Last Update Submitted: 2019-05-16
• Last Update Posted: 2019-05-20

Recruitment & Eligibility

• Status: TERMINATED
• Sex: Male
• Target Recruitment: 37

Inclusion Criteria

• Male
• 70-75 years old
• Signed written informed consent

Exclusion Criteria

• Incompatible with exercise
• Known cardiac disease
• Active cancer
• Significant pulmonary disease
• Uncontrolled hypertension
• Use of blood thinning medicine (except Albyl-E)
• Drug abuse
• Alcohol abuse
• Finishing less than 80% of the exercise sessions (this complies to the training groups)

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
High-intensity interval training	High-intensity interval training	The High-intensity interval training group was instructed to complete exercise sessions of 4x4-minutes intervals at 85-95% of maximal heart rate, 3 times a week for 8 weeks. This type of exercise include 10 minutes warm-up, followed by four intervals of four minutes high-intensity (85-95% of HRmax), with three minutes active breaks (70% of HRmax) between each interval, and ending with seven minutes cool-down. An exercise session last for 42 minutes, where the participants should breathe heavily and feel exhausted four times.
Moderate training	Moderate training	The moderate training group was instructed to complete exercise sessions of 50 minutes at 70% of maximal heart rate, 3 times a week for 8 weeks.

Primary Outcome Measures

Name	Time	Method
Gene Expression	8 weeks
DNA-methylation	8 weeks	From the skeletal muscle biopsies RNA and DNA was isolated, DNA was further bi-sulfite converted and DNA-methylation was analyzed using the Illumina HumanMethylation450 BeadChip. The RNA was further used for gene expression analyses by real-time RTqPCR.

Secondary Outcome Measures

Name	Time	Method
Maximal Aerobic Capacity	8 weeks	measured on treadmill at baseline and after eight weeks, also biopsies from Vastus lateralis were sampled.

Trial Locations

Locations (1)

Institute for Circulation and Medical Imaging, NTNU; 🇳🇴 Trondheim, Norway