The Effect of Exercise on Maximal Aerobic Capacity, DNA-methylation and Gene Expression in Healthy Elderly Men
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Healthy
- Sponsor
- Norwegian University of Science and Technology
- Enrollment
- 37
- Locations
- 1
- Primary Endpoint
- Gene Expression
- Status
- Terminated
- Last Updated
- 6 years ago
Overview
Brief Summary
The purpose of the study was to investigate whether regular moderate- or high-intensity interval training can change the DNA methylation in a long-lasting state, and whether these changes affect the gene expression. In addition, the investigators will determine whether the intensity of the training could be an affecting factor of the possible DNA-methylation change as well. The null hypothesis was that no such exercise-induced changes occur.
Investigators
Eligibility Criteria
Inclusion Criteria
- •70-75 years old
- •Signed written informed consent
Exclusion Criteria
- •Incompatible with exercise
- •Known cardiac disease
- •Active cancer
- •Significant pulmonary disease
- •Uncontrolled hypertension
- •Use of blood thinning medicine (except Albyl-E)
- •Drug abuse
- •Alcohol abuse
- •Finishing less than 80% of the exercise sessions (this complies to the training groups)
Outcomes
Primary Outcomes
Gene Expression
Time Frame: 8 weeks
DNA-methylation
Time Frame: 8 weeks
From the skeletal muscle biopsies RNA and DNA was isolated, DNA was further bi-sulfite converted and DNA-methylation was analyzed using the Illumina HumanMethylation450 BeadChip. The RNA was further used for gene expression analyses by real-time RTqPCR.
Secondary Outcomes
- Maximal Aerobic Capacity(8 weeks)