Exercise-induced Changes in DNA-methylation
- Conditions
- Healthy
- Registration Number
- NCT01931072
- Lead Sponsor
- Norwegian University of Science and Technology
- Brief Summary
The purpose of the study was to investigate whether regular moderate- or high-intensity interval training can change the DNA methylation in a long-lasting state, and whether these changes affect the gene expression. In addition, the investigators will determine whether the intensity of the training could be an affecting factor of the possible DNA-methylation change as well. The null hypothesis was that no such exercise-induced changes occur.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- TERMINATED
- Sex
- Male
- Target Recruitment
- 37
- Male
- 70-75 years old
- Signed written informed consent
- Incompatible with exercise
- Known cardiac disease
- Active cancer
- Significant pulmonary disease
- Uncontrolled hypertension
- Use of blood thinning medicine (except Albyl-E)
- Drug abuse
- Alcohol abuse
- Finishing less than 80% of the exercise sessions (this complies to the training groups)
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Primary Outcome Measures
Name Time Method Gene Expression 8 weeks DNA-methylation 8 weeks From the skeletal muscle biopsies RNA and DNA was isolated, DNA was further bi-sulfite converted and DNA-methylation was analyzed using the Illumina HumanMethylation450 BeadChip. The RNA was further used for gene expression analyses by real-time RTqPCR.
- Secondary Outcome Measures
Name Time Method Maximal Aerobic Capacity 8 weeks measured on treadmill at baseline and after eight weeks, also biopsies from Vastus lateralis were sampled.
Trial Locations
- Locations (1)
Institute for Circulation and Medical Imaging, NTNU
🇳🇴Trondheim, Norway
Institute for Circulation and Medical Imaging, NTNU🇳🇴Trondheim, Norway