The Effect of Exercise on Maximal Aerobic Capacity, DNA-methylation and Gene Expression in Healthy Elderly Men
试验速览
- 阶段
- 不适用
- 状态
- 终止
- 入组人数
- 37
- 试验地点
- 1
- 主要终点
- Gene Expression
研究概览
简要总结
The purpose of the study was to investigate whether regular moderate- or high-intensity interval training can change the DNA methylation in a long-lasting state, and whether these changes affect the gene expression. In addition, the investigators will determine whether the intensity of the training could be an affecting factor of the possible DNA-methylation change as well. The null hypothesis was that no such exercise-induced changes occur.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 70 Years 至 75 Years（Older Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •70-75 years old
- •Signed written informed consent
排除标准
- •Incompatible with exercise
- •Known cardiac disease
- •Active cancer
- •Significant pulmonary disease
- •Uncontrolled hypertension
- •Use of blood thinning medicine (except Albyl-E)
- •Drug abuse
- •Alcohol abuse
- •Finishing less than 80% of the exercise sessions (this complies to the training groups)
研究组 & 干预措施
High-intensity interval training
The High-intensity interval training group was instructed to complete exercise sessions of 4x4-minutes intervals at 85-95% of maximal heart rate, 3 times a week for 8 weeks. This type of exercise include 10 minutes warm-up, followed by four intervals of four minutes high-intensity (85-95% of HRmax), with three minutes active breaks (70% of HRmax) between each interval, and ending with seven minutes cool-down. An exercise session last for 42 minutes, where the participants should breathe heavily and feel exhausted four times.
干预措施: High-intensity interval training (Behavioral)
Moderate training
The moderate training group was instructed to complete exercise sessions of 50 minutes at 70% of maximal heart rate, 3 times a week for 8 weeks.
干预措施: Moderate training (Behavioral)
Control
The control group followed the baseline and follow-up tests, and was asked to live normally and not change their dietary pattern or exercise habits during their participation in this project. Based on the well-known beneficial effects of exercise on general health, it was regarded unethical to demand the control group to desist from any types of physical activity during the project period. They got no further follow-up on exercise.
结局指标
主要结局
Gene Expression
时间窗: 8 weeks
DNA-methylation
时间窗: 8 weeks
From the skeletal muscle biopsies RNA and DNA was isolated, DNA was further bi-sulfite converted and DNA-methylation was analyzed using the Illumina HumanMethylation450 BeadChip. The RNA was further used for gene expression analyses by real-time RTqPCR.
次要结局
- Maximal Aerobic Capacity(8 weeks)