Associations of Vitamin D Deficiency and Vitamin D Receptor Polymorphisms With the Risk of Primary Open-angle Glaucoma

Completed

• Conditions: Vitamin D Deficiency; Primary Open- Angle Glaucoma
• Interventions: Other: Vitamin D receptor polymorphic analysis; Other: Serum levels of 1a, 25-Dihydroxyvitamin D3

• Registration Number: NCT02539745
• Lead Sponsor: Lv Yingjuan

Brief Summary

This study investigated whether vitamin D receptor gene polymorphism is altered in primary open-angle glaucoma subjects carrying the risk allele and vitamin D deficiency is an important factor in the development of glaucoma. Primary open-angle glaucoma patients and age-matched people in the Han population were enrolled. Serum levels of 1a, 25-Dihydroxyvitamin D3 were measured by enzyme-linked immunoabsorbent assay. Vitamin D receptor polymorphic analysis was studied by real-time polymerase-chain reaction high resolution melting analysis.

Detailed Description

Not available

Study Timeline

• Study Start: 2013-07
• Primary Completion: 2014-12
• Study Completion: 2015-03
• Study First Submitted: 2015-08-03
• Study First Submitted QC: 2015-08-31
• Study First Posted: 2015-09-03
• Status Verified: 2016-04
• Last Update Submitted: 2016-04-21
• Last Update Posted: 2016-04-22

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 200

Inclusion Criteria

• intraocular pressure greater than 22 mmHg with two or more medications
• wide anterior chamber angle
• glaucomatous optic neuropathy (Glaucomatous optic nerve damage was defined as cup-to-disc ratio higher than 0.7 or focal loss of the nerve fiber layer (notch) associated with a consistent glaucomatous visual field defect)
• visual field loss consistent with optic nerve damage and visual fields were performed by using standard automated perimetry

Exclusion Criteria

• the presence of any secondary glaucoma including exfoliation syndrome or a history of ocular trauma
• high myopia
• macular degeneration
• other ocular diseases
• a known history of systemic diseases and administration of vitamin D3 or other analog

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
primary open-angle glaucoma patients	Vitamin D receptor polymorphic analysis	Serum levels of 1a, 25-Dihydroxyvitamin D3 were measured by enzyme-linked immunoabsorbent assay and vitamin D receptor polymorphic analysis was studied by real-time polymerase-chain reaction high resolution melting analysis in primary open-angle glaucoma patients.
randomly age-matched people	Serum levels of 1a, 25-Dihydroxyvitamin D3	Serum levels of 1a, 25-Dihydroxyvitamin D3 were measured by enzyme-linked immunoabsorbent assay and vitamin D receptor polymorphic analysis was studied by real-time polymerase-chain reaction high resolution melting analysis in randomly age-matched people.
primary open-angle glaucoma patients	Serum levels of 1a, 25-Dihydroxyvitamin D3	Serum levels of 1a, 25-Dihydroxyvitamin D3 were measured by enzyme-linked immunoabsorbent assay and vitamin D receptor polymorphic analysis was studied by real-time polymerase-chain reaction high resolution melting analysis in primary open-angle glaucoma patients.
randomly age-matched people	Vitamin D receptor polymorphic analysis	Serum levels of 1a, 25-Dihydroxyvitamin D3 were measured by enzyme-linked immunoabsorbent assay and vitamin D receptor polymorphic analysis was studied by real-time polymerase-chain reaction high resolution melting analysis in randomly age-matched people.

Primary Outcome Measures

Name	Time	Method
Serum levels of 1a, 25-Dihydroxyvitamin D3	1 day visit

Secondary Outcome Measures

Name	Time	Method
gene polymorphisms of vitamin D receptor	1 day visit

Trial Locations

Locations (1)

Tianjin Medical University Eye Hospital; 🇨🇳 Tianjin, Tianjin, China