Supplementation of a Leucine-Enriched Dairy Protein Blend: Effects on Plasma Amino Acids in Older Adults
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 8
- 试验地点
- 1
- 主要终点
- Easy-fast amino acid sample testing kit for gas chromatography mass spectrometry
研究概览
简要总结
It is well known that dietary protein is a powerful transient stimulator of the muscle protein synthetic rate (MPS) whereby changes in MPS in response to feeding may be regulated by specific downstream target proteins of mammalian target of rapamycin signaling, such as S6K1, rpS6, and eIF2B. A meal deficient in protein, however, does not increase the rate of MPS because a rise in the bioavailability of amino acids does not occur. In addition, the source of dietary proteins has been shown to impact postprandial blood levels of amino acids. The concept that certain types of proteins are "fast acting" or "slow acting" has been shown to affect the postprandial profile of amino acids appearing in the systemic circulation. Native whey and micellar casein are both dairy proteins that contain a similar amount of essential (EAA), but blood EAA levels increase faster and to a higher level after the consumption of whey protein. Differences in gastric emptying, digestion and absorption kinetics between micellar casein and native whey are the underlying factors. Nonetheless, micellar casein protein has been shown to protract MPS in humans. Despite the significant amount of information gained with respect to both of these protein sources, the effects of combinatorial formulations on the postprandial profile of amino acids appearing in the blood is less well known. The purpose of the present study is to determine post-ingestion aminoacidemia, glycemia, and insulinemia from a specially formulated dairy protein blend.
详细描述
Experimental approach:
8 participants will be included in this study. Each participant will consume the supplement only once in a randomized fashion during three separate visits and there will be approximately 1 week between each visit.
Standardized meal:
Participants will be provided a standardized diet one day prior to experimentation. When performing research involving human metabolism it is important that the participants be tested in a weight-stable state. Therefore, the investigators will estimate the participants resting energy expenditure using a widely reference prediction formula, known as the Harris-Benedict equation. This formula takes into account gender, body mass, height, age, and self-reported activity level. Once resting energy expenditure is calculated the investigators will provide each participant with a pre-packaged standardized diet for the day prior to the experiment that is designed to ensure a sufficient energy balance.
Dual Energy X-Ray Absorptiometry (DXA) Scans:
研究设计
- 研究类型
- Observational
- 观察模型
- Case Crossover
- 时间视角
- Prospective
入排标准
- 年龄范围
- 65 Years 至 —（Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •8 participants will be included in this study. Each participant will consume the supplement only once in a randomized fashion during one of three separate visits and there will be approximately 1 week between each visit. In order to participate in this study, each participant must be male or female, 65 years or older (inclusive) and cannot be a smoker or user of tobacco products.
排除标准
- •The exclusion requirements for this study include the following conditions:
- •Take any analgesic or anti-inflammatory drugs(s), prescription or non-prescription, chronically will be excluded. However, a washout period of 4 weeks will be suitable for participation.
- •A history of neuromuscular problems or muscle and/or bone wasting diseases
- •Any acute or chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin- or non-insulin dependent diabetes or other metabolic disorders-all ascertained through medical history screening questionnaires
- •Use medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications)
结局指标
主要结局
Easy-fast amino acid sample testing kit for gas chromatography mass spectrometry
时间窗: 375 minutes
plasma amino acid concentrations
次要结局
- Hexokinase/G-6-PDH methodology(375 minutes)
- Visual Analog Scale Questionnaires for food sensory perception for taste(Average score across 2 measurements)
- Visual Analog Scale Questionnaires for food sensory perception for pleasantness(Average score across 2 measurements)
- Visual Analog Scale Questionnaires for food sensory perception desire to eat(Average score across 13 measurements)
- Visual Analog Scale Questionnaires for food sensory perception for hunger(Average score across 13 measurements)
- Visual Analog Scale Questionnaires for food sensory perception for smell(Average score across 2 measurements)
- Visual Analog Scale Questionnaires for food sensory perception for how much food could be eaten(Average score across 13 measurements)
- Visual Analog Scale Questionnaires for food sensory perception for liking or disliking of food(Average score across 13 measurements)
- Visual Analog Scale Questionnaires for food sensory perception for fullness(Average score across 13 measurements)
- Visual Analog Scale Questionnaires for food sensory perception for visual appeal(Average score across 2 measurements)
- Chemiluminescent Microparticle Immunoassay(375 minutes)
- Visual Analog Scale Questionnaires for food sensory perception for thirst(Average score across 13 measurements)
- Visual Analog Scale Questionnaires for food sensory perception for aftertaste(Average score across 2 measurements)
- Height in meters(One measurement at baseline)
- Weight in kilograms(One measurement at baseline)
研究者
Stuart Phillips
Professor
McMaster University