Utilizing Free DNA in Embryo Culture for PGT
Completed
- Conditions
- Genetic Diseases, InbornMutationChromosome Abnormalities
- Interventions
- Procedure: NGS reading for Media Free DNA
- Registration Number
- NCT02884063
- Lead Sponsor
- Al Baraka Fertility Hospital
- Brief Summary
In the way for developing and optimizing protocol to be used as non- invasive methodology used as routine testing for PGS.
This protocol is to be adapted to replace the using of life embryo cells for genetic testing and aneuploidy study as well as for any type of genetic testing including single gene disorder or HLA typing or study.
- Detailed Description
Compare 100 embryos studied using NGS for chromosomal aneuploidies, and compare the results came from free media DNA with this results.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 100
Inclusion Criteria
- all abnormal/aneuploidy embryos rejected for embryo transfer.
Exclusion Criteria
- all embryos had unsuccessful diagnosis with blastomere biopsy.
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description NGS reading for blastomer DNA NGS reading for Media Free DNA DNA extracted from Blastomere used for aneuploidy screening. NGS reading for Media Free DNA NGS reading for Media Free DNA Ability to read the DNA product available in embryo culture media using NGS to have valuable diagnostic image for genetic composition of embryo before implantation.
- Primary Outcome Measures
Name Time Method DNA isolation 2 years reading amplified DNA using NGS
- Secondary Outcome Measures
Name Time Method Comparison with cell reading using NGS 2 years compare the obtained result with the results provided collected for the same embryo using its blastomer for PGS
NGS evaluation 2 years Free DNA isolation and amplification using WGA