NCT02884063
Completed
Not Applicable
Utilizing Free DNA in Embryo Culture Media for Preimplantation Genetic Screening
Al Baraka Fertility Hospital0 sites100 target enrollmentMay 2015
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Chromosome Abnormalities
- Sponsor
- Al Baraka Fertility Hospital
- Enrollment
- 100
- Primary Endpoint
- DNA isolation
- Status
- Completed
- Last Updated
- 4 years ago
Overview
Brief Summary
In the way for developing and optimizing protocol to be used as non- invasive methodology used as routine testing for PGS.
This protocol is to be adapted to replace the using of life embryo cells for genetic testing and aneuploidy study as well as for any type of genetic testing including single gene disorder or HLA typing or study.
Detailed Description
Compare 100 embryos studied using NGS for chromosomal aneuploidies, and compare the results came from free media DNA with this results.
Investigators
Ahmad Mustafa Mohamed Metwalley
IVF laboratory manager
Al Baraka Fertility Hospital
Eligibility Criteria
Inclusion Criteria
- •all abnormal/aneuploidy embryos rejected for embryo transfer.
Exclusion Criteria
- •all embryos had unsuccessful diagnosis with blastomere biopsy.
Outcomes
Primary Outcomes
DNA isolation
Time Frame: 2 years
reading amplified DNA using NGS
Secondary Outcomes
- NGS evaluation(2 years)
- Comparison with cell reading using NGS(2 years)
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