Study of Free DNA Content of Embryo Culture Medium as a Non-Invasive Tool for Embryo Assessment

Completed

• Conditions: Infertility
• Interventions: Other: measuring cell freeDNA

• Registration Number: NCT02480491
• Lead Sponsor: Sadat City University

Brief Summary

Study of Cell Free DNA Content of Human Embryo Culture Medium as a Non-Invasive Tool for Embryo Assessment in intra cytoplasmic sperm injection Treatment Cycles.

Detailed Description

The aim of the present study is to determine whether cell free DNA content of the culture medium of the human embryo correlates with the quality and reproductive potential of embryos in ICSI cycles and, thus, to look for a new non invasive tool of assessing embryo quality beside morphological assessment. Determining a rapid, noninvasive method for the assessment of the developmental potential of embryos may be a great achievement

Study Timeline

• Study Start: 2014-01
• Primary Completion: 2015-02
• Study Completion: 2015-05
• Status Verified: 2015-06
• Study First Submitted: 2015-06-20
• Study First Submitted QC: 2015-06-23
• Last Update Submitted: 2015-06-23
• Study First Posted: 2015-06-24
• Last Update Posted: 2015-06-24

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 100

Inclusion Criteria

• third day embryos

Exclusion Criteria

• less than third day embryos

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Fifth day embryos	measuring cell freeDNA	embryos culture media during fifth day after fertilization
Third day embryos	measuring cell freeDNA	embryos culture media during third day after fertilization

Primary Outcome Measures

Name	Time	Method
correlations between levels of measured cell free DNA and embryo quality.	12 months