Recovery Kinetics Following a Soccer Training in Middle-aged Males

Not Applicable

Recruiting

• Conditions: Skeletal Muscle Damage
• Interventions: Other: ST

• Registration Number: NCT06260215
• Lead Sponsor: University of Thessaly

Brief Summary

This study aims at investigating the recovery kinetics of skeletal muscle damage, neuromuscular fatigue and performance following a single soccer trainning session in middle aged males. The participants will perform a soccer training session \[A single training session including 60 minutes of warm up, soccer technical exercises and small-sided game\] and a cotrol trial (No intervention included, only daily measurements) in randomized, repeated measures, crossover design. Assesesments related to skeletal muscle damage, performance and neuromuscular fatigue will be executed before the training session and daily for four consecutive days after training.

Detailed Description

Eleven male middle-aged participants will be included in this study. Participants will be initially informed about the main goals of study as well as the associated risks and benefits and then will provide their signed consent form. At baseline, they will undergo assessment of their anthropometrics (body mass, height, BMI, WHR), body compotition (using DXA), physical contition level (peak oxygen consumption, VO2peak), haemodynamic profile (diastolic and diastolic blood pressure) and daily dietary intake.Thereafter, they will participate in two experimental trials (Control trial and Soccer trial) in a randomized, crossover, repeated measure design: 1) ST (Soccer Trial): Participants in this trial will perform a soccer training session (60 min) consisted of warm-up, technical exercises and small-sided game, CT (Control Trial): Participants in this trial will only execute the evaluations daily in the dependent variables (they will not receive any intervention). Before each trial, the participants will provide a resting blood sample (for the determination of white blood cells, granulocyte cells, monocyte cells, lymphocyte cells, Hemeatocrit, Hemoglobin, red blood cells and platelets, total antioxidant capacity, glutathione and creatine kinase activity) and undergo assessments of their delay onset muscle soreness (DOMS), maximal voluntary isometric contraction (MVIC) of knee extensors and flexors (by isokinetic dynamometer), height and power of countermovement jump (CMJ-using force platform), 10m and 30m sprint time (will be evaluated using light cells). A 7-day wash-out period will apllied between ST and CT. The blood sample will be collected before and 24, 48 and 72 hours post-taining. Also, the evaluation of DOMS, MVIC, sprinting performance, power and jump height (by CMJ) will be evaluated before and 24, 48, 72 and 96 hours post training.

Study Timeline

• Study First Submitted: 2024-01-29
• Status Verified: 2024-02
• Study Start: 2024-02-05
• Study First Submitted QC: 2024-02-06
• Study First Posted: 2024-02-15
• Last Update Submitted: 2024-02-19
• Last Update Posted: 2024-02-21
• Primary Completion: 2024-02-26
• Study Completion: 2024-03-09

Recruitment & Eligibility

• Status: RECRUITING
• Sex: Male
• Target Recruitment: 11

Inclusion Criteria

• Free of musculoskeletal injuries
• No use of ergogenic supplements or medication
• Free of chronic diseases
• age of 40-60 years

Exclusion Criteria

• Musculoskeletal injury
• Use of alcohol, caffeine and any type of ergogenic supplements or medication during the course of the study

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
ST	ST	Participants in this arm will participate in a soccer training (ST) session for 60 minutes. During the training the participans will execute the warm-up process, the part of soccer technical exercises and they will play a small-side-game.

Primary Outcome Measures

Name	Time	Method
Change in Granulocyte cell count	At baseline, at 24, 48 and 72 hours after soccer training	Change in White blood cell count will be measured using an automatic blood analyzer
Change in Platelets cell count	At baseline, at 24, 48 and 72 hours after soccer training	Platelets cell count will be measured using an automatic blood analyzer
Change in Lymphocyte cell count	At baseline, at 24, 48 and 72 hours after soccer training	Lymphocyte cell count will be measured using an automatic blood analyzer
Change in Monocyte cell count	At baseline, at 24, 48 and 72 hours after soccer training	Monocytes cell count will be measured using an automatic blood analyzer
Change in Glutathione concentration in blood	At baseline, at 24, 48 and 72 hours after soccer training	Glutathione concentration will be measured in red blood cells
Change in Peak power during coutermovement jump test	At baseline, at 24, 48, 72 and 96 hours after soccer training	The peak power will be measured using a force platform
Change in countermovement jump height	At baseline, at 24, 48, 72 and 96 hours after soccer training	countermovement jump height will be measured using a force platform
Change in isometric peak torque of knee extensors and flexors	At baseline, at 24, 48, 72 and 96 hours after soccer training	Isometric peak torque will be assessed on an isokinetic dynamometer
Change in White blood cell count	At baseline, at 24, 48 and 72 hours after soccer training	White blood cell count will be measured using an automatic blood analyzer
Change Red blood cells count	At baseline, at 24, 48 and 72 hours after soccer training	Red blood cells count will be measured using an automatic blood analyzer
Change in Creatine kinase activity	At baseline, at 24, 48 and 72 hours after soccer training	Creatine kinase activity will be measured in plasma
Change in Total antioxidant capacity	At baseline, at 24, 48 and 72 hours after soccer training	Total antioxidant capacity will be measured in plasma
Change in delayed onset of muscle soreness	At baseline, at 24, 48, 72 and 96 hours after soccer training	Muscle soreness will be assessed by palpation of muscle belly and the distal region
Change in percentage of Hematocrit level	At baseline, at 24, 48 and 72 hours after soccer training	The percentage of Hematocrit level will be measured using an automatic blood analyzer
Change in Hemoglobin level	At baseline, at 24, 48 and 72 hours after soccer training	Hemoglobin level will be measured using an automatic blood analyzer
Change in sprint time of 10 m	At baseline, at 24, 48, 72 and 96 hours after soccer training	10m sprint time will be assessed using light cells
Change in sprint time of 30 m	At baseline, at 24, 48, 72 and 96 hours after soccer training	30m sprint time will be assessed using light cells

Secondary Outcome Measures

Name	Time	Method
Body Mass	At baseline	Body Mass will be measured on a beam balance with stadiometer
Body Fat	At baseline	Body Fat will be measured by using Dual-emission X-ray absorptiometry
Bone mass content	At baseline	Bone mass content will be measured by using Dual-emission X-ray absorptiometry
Field activity during the soccer training	During the 60 minutes soccer training (ST) session (72 hours after baseline measurements)	The field activity will be continuously monitored during the soccer training using global positioning system (GPS)
Bone mass density	At baseline	Bone mass density will be measured by using Dual-emission X-ray absorptiometry
Dietary intake	At baseline	Dietary intake will be assesed over a 7-day period using diet recalls
Lean body mass	At baseline	Lean body mass will be measured by using Dual-emission X-ray absorptiometry
Heart rate during the soccer training	During the 60 minutes soccer training (ST) session (72 hours after baseline measurements)	Heart rate will be continuously monitored during the soccer training using heart rate monitors
Systolic Blood Pressure	At baseline	Systolic Blood Pressure will be examined using sphygmomanometer
Rest Heart Rate	At baseline	The rest heart rate will be estimated using a heart rate monitor
Peak Maximal oxygen consumption (Peak VO2)	At baseline	Peak VO2 will be estimated by open circuit spirometry via breath by breath method
Body Height	At baseline	Body Height will be measured on a beam balance with stadiometer
Dystolic Blood Pressure	At baseline	Dystolic Blood Pressure will be examined using sphygmomanometer

Trial Locations

Locations (1)

University o Thessaly, School of Physical Education and Sports Science; 🇬🇷 Tríkala, Greece